Last Updated on Sep 2021
•
Switch on the argon laser
•
Open LAS X program
•
Confirm laser emission
•
Gating function for removing autofluorescence photons
only works for pulsed lasers (WLL). It will not work for
continuous mode argon lasers.
No signal viewed from
the sample
Check that your sample does actually have the signal using the
Wide Field eyepiece.
If there is signal from the Wide Field eyepiece:
1.
No laser emission
•
Refer to previous items
2.
Wrong detector settings
•
Check detector settings on LAS X
3.
Hybrid Detector overloaded
•
Look at the bottom of the LAS X window. If it says the
HyD overloaded, use the following steps:
1)
Turn off Live View.
2)
Ensure that the detection band is set at least 10nm
away from any laser you are using.
3)
Set the HyD gain to 100% always. Brightness should be
set using the laser power only for best sensitivity and
minimal photobleaching risk
4)
Drop the laser power to the lowest level, then switch
on Live View.
5)
On Live View, carefully increase the laser power using
the mouse wheel until you can see the sample on Live
View.
6)
Watch the information ticker in case the overload
message appears again.
4.
Out of focus
•
Confocal microscopes are very sensitive to focus shifts.
Use the panel knob to carefully shift focus in and out
until you see a signal.
*Refer to Troubleshooting Appendix for location of switches
