Aperio AT2 DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019
42
Chapter 7: Quality Control and Maintenance
Do this procedure for each magnification level, setting the magnification level each time.
If the scanner cannot create a calibration image after setting the line camera gains, the LED light source may need to be
replaced or adjusted—contact your Leica Biosystems Technical Services representative for assistance.
Clean the Objective
We recommend cleaning the objective every month. To protect the objective, use gloves when handling it. If you are
removing the objective to clean it, store the objective in the objective case supplied by Leica Biosystems when it is outside
of the scanner.
Because the tip of the objective is more scratch resistant than the interior lens, first try cleaning the objective without
removing it from the scanner.
Ì
`
To avoid the possibility of introducing very fine scratches onto the surface, never drag anything across the lens
surface with a high degree of pressure, including lens paper.
Ì
`
Clean the lens with a solvent designed for optical surfaces or, in an emergency, absolute ethanol. Avoid other
solvents because they might react with optical coatings on the glass.
Ì
`
Slightly moisten a Q-tip with lens cleaner or ethanol and very gently wipe it over the lens several times, turning the
cotton tip before each pass. Blot excess solvent with lens tissue and allow the lens to dry thoroughly. Repeat this
procedure if necessary.
Removing the Objective
If cleaning the objective in the scanner does not fully remove artifacts, you will need to remove the objective and clean it
more thoroughly outside of the scanner.
To remove the objective, place two fingers of one hand under the objective to protect it and using your other hand carefully
unthread the objective rotating to the left (or counterclockwise if viewing from below) without touching the glass lens as
you rotate it. When the objective is free of the mount, remove the objective and place it in an objective case to protect it.
After you remove the objective, you may see a Console error message that a motion error has occurred—ignore that
message for now and continue cleaning the lens.
Dust the lens surface with compressed air prior to cleaning to remove loose particles. Only use compressed air if the
objective has been removed from the scanner—never use compressed air inside the scanner.
Now follow the cleaning steps in the section above.
Installing the Objective
To install the objective into the scanner after cleaning it, while keeping two fingers under it without touching the glass lens,
use your other hand to carefully hold the objective by its sides, seat it in the objective mount, and rotate it to the right (or
clockwise if viewing from below) to secure it. Do not cross-thread or over tighten.
After you install the objective, the scanner Console may display a message that a motion error has occurred. While changing
the objective, you may have moved the stage which causes a motion error. Power down the scanner and restart the
Controller to rehome the stage and return it to a known position. See
“Recovering from a Motion Fault” on page 55
for
more information. For more information on cleaning objectives, please see:
http://www.olympusmicro.com/primer/techniques/fluorescence/troubleshoot.html
