8
Low Pressure Gradient (LPG) Block
Please make sure that the solvent bushings and the blind fittings
are tight. If they are not, the specifications of the LPG cannot be
guaranteed.
Set the desired low-pressure gradient in the pump’s setup menu
according to SOP 1and SOP 2.
SOP 1
Selecting a Gradient Mode
This SOP applies to the Smartline Pump 1000 Firmware Revision 1.0X.
Use this SOP for selecting a gradient mode.
1. Select „GRADIENT MODE“ in the SETUP Menu.
2. Select the desired mode of operation.
3. If you select either high or low pressure gradient (i. e. HPG or LPG)
you need to specify the channels of used solvents:
Low Pressure Gradient: Choose valves A to D
High Pressure Gradient: Choose pump names HPG A to HPG D
4. Move to the second line by pressing
¾
and choose valves A to D
(for LPG mode) or pumps actually present (for HPG mode) on the
corresponding positions by pressing
¿
or
À
. This selection will
apply for programs and define the number and names of solvents
used in any gradient.
SOP 2
Selection of operation mode:
This SOP applies to the Smartline Pump 1000 Firmware Revision 1.0X.
1. Return to page GRADIENT MODE by pressing
¿
.
2. Press
¾
and then
¿
to scroll through the gradient mode options
until LPG appears.
3. Use
¾
to position cursor in the second line.
4. Select ON or -- (off) at positions A, B, C and D for the solvent
channels in either mode you wish to use. The number of ONs
selected refers to all programs in the pumps memory.
5. At any position you can use
¿
or
À
to select ON.
6. Position cursor on next position, e. g. B and select ON .
7. Select three times
¾
to position cursor at the rhombus.
8. The display should appear as shown in Figure 3.
9. Leave this menu page with
½
to return to the main menu.
GRADIENT MODE: LPG
ON
ON
--
--
Load
Edit Prog
Flow
Pressure
Events
Prog
Clear 0
[ml/min]
[0.1 Mpa]
off 0
on 1
2
Hold 0
Time
%A
%B
%C
%D
Run 1
[min]
Setup
GLP
Link
View
Figure 3
Example for a gradient setup with solvents A and B in all programs
The LPG block and the connection tubes should be rinsed with the
provided solvent and filled before they are used.
When the pump is turned off, the LPG block’s valves are closed. To
rinse the LPG block, set the flow rate to 0 ml/min and set the
pertinent mixture portion of the solvent for the respective valve to