Knauer AZURA P 2.1S User Manual Download

Page: 9 / 47

Scope of Delivery

P 2.1S/P 4.1S user manual V6870, version 3.1

Scope of Delivery

Note:

Only use original parts and accessories made by KNAUER or a company

authorized by KNAUER.



Pump P 2.1S/P 4.1S



User manual



Power adapter 24 V



Installation Qualification Document



Accessories Kit Pump



Accessories Kit AZURA

Safety

Professional Group

The user manual addresses persons who are qualified as chemical laboratory
technicians or have completed comparable vocational training.

The following knowledge is required:



Fundamental knowledge of liquid chromatography



Knowledge regarding substances that are suitable only to a limited extent for
use in liquid chromatography



Knowledge regarding the health risks of chemicals



Participation during an installation of a device or a training by the company
KNAUER or an authorized company.

If you do not belong to this or a comparable professional group, you may not
perform the work described in this user manual under any circumstances. In this
case, please contact your superior.

Safety Equipment

When working with the device, take measures according to lab regulations and
wear protective clothing:



Safety glasses with side protection



Protective gloves



Lab coat



Dilute acetic acid (10-50%), at 25° C/77.0° F



Dilute sodium hydroxide (1M)



Water

1. valid for the specified temperature range

Suitable eluents

Summary of Contents for AZURA P 2.1S

Page 1: ...Pump P 2 1S P 4 1S User Manual V6870 HPLC ...

Page 2: ...Note The contents of this user manual apply for the BlueShadow product as well Please submit any request on article numbers for BlueShadow ...

Page 3: ...igns 12 Unpacking and Setup 12 Contacting the Technical Support 12 Location Requirements 12 Requirements 12 Power supply 13 Unpacking 13 Connecting the Eluent Line to the Pump Head 14 Control 15 Pin Header for Remote Control 15 Computer Control 17 Configuring the LAN Settings 17 Connecting the Cables 18 Configuring the Router 19 Integrating the LAN into a Company Network 19 Controlling Several Sys...

Page 4: ...act 35 Pump Head 35 Removing the Pump Head 35 Installing the Pump Head 36 Check Valves 36 Removing the Check Valves 37 Cleaning the Check Valves 37 Installing the Check Valves 38 Technical Data 39 Accessories and Spare Parts 40 Device and Accessories 40 Device Variations 40 Available Pump Heads 41 Legal Information 41 Transport Damage 41 Warranty Conditions 41 Declaration of Conformity 42 Disposal...

Page 5: ...ail Device Overview The HPLC pumps P 4 1S P 2 1S with pump heads can be used as feed pumps or dosing pumps in analytical or preparative applications Pumps traP 2 1S P 4 1S user manual V6870 version 3 1nsport solvents or dissolved samples through the HPLC system Fig 1 P 2 1S with 10 ml pump head Fig 2 P 4 1S with 10 ml pump head Legend 1 Display 2 Keypad 3 Pump head 4 Pressure sensor Fig 3 Pump rea...

Page 6: ...to 150 bar Dual piston technology for constant flow rates Setting a limit for minimum and maximum pressure to protect the columns and to avoid a dry run of the pump only P 4 1S Emergency stop independent from control with chromatography software The pump can be controlled with the keypad in standalone mode or with the chromatography software The pump heads can be easily removed and replaced via fo...

Page 7: ...for use in analytical applications Stainless steel with stainless steel inlays for standard applications Stainless steel with ceramics inlays for biocompatibility Stainless steel with Hastelloy C inlays for aggressive media different pump head sizes 10 ml or 50 ml The front of the pump head is labeled with the specifications for the maximum pumping capacity 10 ml or 50 ml Pump heads with inlays ca...

Page 8: ...ents Halogenated hydrocarbons e g Freon Concentrated mineral and organic acids Concentrated bases Eluents containing particles Perfluorinated eluents e g Fluorinert FC 75 FC 40 Perfluorinated polyether e g Fomblin Less suitable eluents Dimethyl sulfoxide DMSO Slightly volatile eluents Methylene chloride Tetrahydrofuran THF Dilute phosphoric acid Suitable eluents Acetone at 4 25 C 39 2 77 0 F 1 Ace...

Page 9: ... regarding substances that are suitable only to a limited extent for use in liquid chromatography Knowledge regarding the health risks of chemicals Participation during an installation of a device or a training by the company KNAUER or an authorized company If you do not belong to this or a comparable professional group you may not perform the work described in this user manual under any circumsta...

Page 10: ...t is prohibited to have any open flames near the analytical system solvent tray Risk of electrical shock or short circuit if liquids get into the device s interior For this reason place all bottles in a solvent tray solvent lines Install capillaries and tubing in such a way that liquids cannot get into the interior in case of a leak leaks Regularly check if any system components are leaking power ...

Page 11: ...ard for all persons during operation repair sale and disposal of a device Life threatening injuries Health danger if getting in contact with toxic infectious or radio active sub stances Before disposing of the device or sending it away for repair you are required to decontaminate the device in a technically correct manner All contaminated devices must be properly decontaminated by a specialist com...

Page 12: ...ion for the device must meet the following requirements Device defect The device overheats at exposure to sunlight and insufficient air circulation Device failures are very likely Set up the device in such a way that it is protected against exposure to direct sunlight Keep at least 15 cm clear at the rear and 5 10 cm at each side for air circula tion Symbol Meaning Electric shock hazard Electrosta...

Page 13: ... packing list carefully for repeat orders Bruising danger Damage to the device by carrying or lifting it on protruding housing parts The device may fall and thus cause injuries Lift the device on the side of the housing only Process 1 Setup the delivery so you are able to read the label Using the utility knife cut the adhesive tape Open the delivery 2 Remove the foam insert Take out the accessorie...

Page 14: ...k the scope of delivery 8 Remove the protective foil Result The device is complete and ready for use Next steps Connect the device to power supply to prepare the initial startup Connecting the Eluent Line to the Pump Head Prerequisite The device has been switched off The power plug has been pulled Tools Flangeless fitting Device defect The pump head can get damaged when the inlet and outlet are bl...

Page 15: ... number 4 RS 232 port 5 LAN port 6 Pin header for remote control 7 Power connection bushing 8 Warning 1 9 Hole for the ground connection Contact data Explanation GROUND Reference point of the voltage at the signal inputs START IN TTL compatible input min 10 mA Low active After receiving a signal from an external device the device starts If controlled with software an electronic trigger is send thr...

Page 16: ...ting cables to the multi pin connector of a switched on device causes a short circuit Turn off the device before connecting cables Pull the power plug Electronic defect Electrostatic discharge can destroy the electronics Wear a protective bracelet against electrostatic discharge and ground ERROR IN OUT TTL compatible input min 10 mA Low active After receiving a signal e g short circuit to ground f...

Page 17: ...ter 3 On the computer configure the router to set up the network 4 Install the chromatography software from the data storage device 5 Switch on the device and run the chromatography software Configuring the LAN Settings The LAN uses only one server which is normally the router from that the devices automatically receive their IP address Prerequisite In Windows power saving hibernation standby and ...

Page 18: ... Check the settings in the tab General The correct settings for the DHCP cli ent are a Obtain IP address automatically b Obtain DNS server address automatically 7 Click on the button OK Connecting the Cables A router 3 has several LAN ports 2 and one WAN port 4 that can be used to integrate the LAN into a wide area network WAN e g a company network or the Internet In contrast the LAN ports serve t...

Page 19: ...igned IP addresses to all devices the chromatography software can be used to remotely control the system Integrating the LAN into a Company Network A network administrator can integrate the LAN into your company network In this case you use the WAN port of the router Prerequisite There is a patch cable for the connection Process 1 Check that the IP address range of the router and of the company ne...

Page 20: ...al Support of KNAUER Never connect the device to the power connection The ground connection for the pump has a designated hole with a thread M3 on the back of the device If the supplied power adapter is used then the ground connection remains unused Please contact the technical support of KNAUER if the pump along with other devices should be connected to the power supply with a 6 prong power adapt...

Page 21: ...he display shows vertical arrows while the pump is flushing Prerequisites Pump with pressure sensor Venting screw of the pressure sensor is open Syringe is connected to venting capillary Pump without pressure sensor Blind fitting in the outlet to the pressure sensor is removed Syringe is connected to the capillary on the pump head outlet Note The flow rate can be changed while being purged The cha...

Page 22: ...itoring the device or changing the settings Setting the Flow Rate In case of the pump without pressure sensor the actual required flow rate is dependent on the resulting counter pressure The absolute deviation is depen Process Figure 1 Prime liquid with the syringe 2 Hold down start stop key until flushing starts Fig 9 Status display Legend 1 Pressure in 0 1 MPa 2 Flow rate in ml min Figure Functi...

Page 23: ... to avoid damaging the pump or pump head Set minimum pressure to avoid running the pump dry Note If the minimum is set to 0 the minimum pump pressure is not moni tored Process Figure 1 Use the arrow keys to set a value for the flow rate 2 Check if the right value appears on the display Fig 12 Display control Legend 1 Maximum value 2 Minimum value Process Figure 1 Press both arrow keys simultane ou...

Page 24: ...e sensor to limit the pump pressure Set the minimum power consumption so as to avoid a dry run of the pump at highly reduced maximum power consumption e g if leaking The pump is preset to a standard value for the maximum power consumption KNAUER recommends that with smaller flow rates the standard value for the maximum power consumption should be insignificantly decreased Note If the minimum is se...

Page 25: ...RS232 cable or a LAN cable 3 When the cursor flashes use the arrow keys again to set the value for the maximum power consump tion 4 Hold down left arrow key Press right arrow key once Fig 15 Minimum power consumption 5 When the cursor flashes use the arrow keys again to set the value for the minimum power consump tion 6 Press start stop key once to return to the start display Fig 16 Maximum power ...

Page 26: ...lay appears Control commands The control commands listed below are considered for the communication with RS 232 and LAN When entering a parameter you must place a colon or space between command and parameter value e g PMIN10 100 Consider the following specifications for data transfer with RS 232 interface 9600 baud 8 bit 1 stop bit no parity check Process Figure 1 Press both arrow keys simultane o...

Page 27: ...WR 0 400 Maximum pressure for 10 ml pump head in 0 1 MPa PMAX50 RD WR 0 150 Maximum pressure for 50 ml pump head in 0 1 MPa IMIN10 RD WR 0 100 Minimum motor current for 10 ml pump head IMIN50 RD WR 0 100 Minimum motor current for 50 ml pump head IMAX10 RD WR 0 100 Maximum motor current for 10 ml pump head IMAX50 RD WR 0 100 Maximum motor current for 50 ml pump head HEADTYPE RD WR 10 50 Writing rea...

Page 28: ...Control firmware version 1 37 or higher If you change the pump to analog control you are changing the start settings for switch on In analog operationg the pump starts with activated flow The flow rate is defined by the applied voltage Close the contact between GROUND and START IN before starting the pump EXTFLOW RD IMOTOR RD 0 100 Motor current in relative units LOCAL WR Put the instrument in loc...

Page 29: ... 1 as parameter for EXTCONTR Controlling the Flow Rate To control the flow rate by an external control voltage you need to select ANA LOG control in the menu Prerequisite The pump is connected to the power supply Process 1 Apply control voltage 2 Press the Start Stop button so as to start the pump The star symbol on the display of the pump indicates that the pump is working with an externally cont...

Page 30: ...atus Process 1 Connect the pump to a suitable terminal program 2 Connect the pump using a LAN or RS 232 3 Enter STARTLEVEL 1 Starting Directly after Connecting to Power Supply By default the pump is stopped and started using the start stop button The STARTMODE setting allows you to start the device right after connecting it to power supply STARTMODE 0 default setting RS 232 LAN The pump does not s...

Page 31: ...R performs this functionality test during the installation The Installation Qualification is a standardized document that comes as part of the delivery and includes the following confirmation of flawless condition at delivery check if the delivery is complete certification on the functionality of the device Operation Qualification OQ The Operation Qualification includes an extensive functionality ...

Page 32: ...ction in the Windows task bar Connected Connection not established If no connection was established test the following Is the router switched on Is the patch cable connected correctly to the router and the computer 2 Check the router settings Is the router set to DCHP server Is the IP address range sufficient for all the connected devices 3 Check all connections Are the patch cable connected to th...

Page 33: ...on the pressure sensor must be turned up Check if the venting screw on the pressure sensor is turned open Pump does not transport solvent Check the following options Purge the pump head so as to remove the air bub bles Inspect the eluent inlet and filter of the HPLC column and change when blocked Replacing the pump head Clean the check valves Exchange the check valves If the pump head seals are de...

Page 34: ...Pump head leaks Check the following options Inspect the inlet and outlet screw fittings of the pump head Replacing the pump head If the pump head seals are defective solvent enters the piston backflushing inform the technical support of KNAUER Flow rate is not cor rect Check the following options Inspect the inlet and outlet screw fittings of the pump head Clean the check valves Exchange the check...

Page 35: ...lts Maintenance Contract The following maintenance work on the device may only be performed by KNAUER or a company authorized by KNAUER and is covered by a separate maintenance contract Opening the device or removing housing parts Pump Head Removing the Pump Head Prerequisite The pump head has been flushed with suitable solvent Chemical burns Aggressive or toxic solvent residue can irritate the sk...

Page 36: ...ular flow Note Insert the valves in the direction of flow Process Figure 1 Unscrew inlet fitting 2 and outlet fitting 1 2 Unscrew the inlet fitting 5 to the eluent 3 Unscrew the outlet fitting 4 to the pressure sensor 4 Alternately unscrew the 4 fastening screws 3 5 Hold the pump head and consecu tively pull out all fastening screws Process Figure 1 Insert the fastening screws 3 and tighten altern...

Page 37: ...size 13 Next steps Clean the check valves Cleaning the Check Valves 1 Fill a beaker with solvent 2 Place the valve in the beaker 3 Put the beaker in an ultrasonic bath for at least 10 minutes Fig 24 Check valve Process Figure 1 Unscrew and remove the capillary connector 1 2 Loosen the outlet fitting 2 with the open end wrench 3 Remove the first check valve 4 Loosen the inlet fitting 3 with the ope...

Page 38: ...erequisite The check valves have dried Device defect Damage to the pump head caused by overtightened capillary fittings Note the torque of the fittings Use 5 Nm torque for stainless steel fittings Use 1 Nm torque for PEEK fittings Next steps Re install the pump head Process Figure 1 Insert the check valves 1 in such a way that the notch of the check valve 2 points downward 2 Screw in inlet and out...

Page 39: ...flow range using ethanol water 10 90 For pumps without a pressure sensor dependent on pressure Flow rate precision Relative standard deviation RSD 0 5 1 ml min Gradients Isocratic HPLC pump Expandable to a binary high pres sure gradient system controlled by software System protection Pump with pressure sensor Pmin and Pmax adjustable Imin and Imax adjustable Pump with pressure sensor Imin and Imax...

Page 40: ... 138 1 227 8 mm width x height depth Weight Pump without pressure sensor 2 3 kg Pump with pressure sensor 2 4 kg Height above sea level maximum 2000 m Name Order number Accessories kit AZURA FZA01 Accessories kit P 2 1S P 4 1S FPGA User manual V6870 Mobile Control license with 10 touchscreen A9607 Mobile Control Chrom license with 10 touchscreen A9608 Mobile Control license A9610 Mobile Control Ch...

Page 41: ...o the device This warranty also excludes the following accidental or willful damage damage or errors caused by third parties that are not contractually related to the manufacturer at the time the damage occurs wear parts fuses glass parts columns light sources cuvettes and other opti cal components damage caused by negligence or improper operation of the device and dam age caused by clogged capill...

Page 42: ...4 WEEE Registration KNAUER as a company is registered by the WEEE number DE 34642789 in the German Elektroaltgeräteregister EAR The number belongs to category 8 and 9 which among others comprise laboratory equipment All distributors and importers are responsible for the disposal of old devices as defined by the WEEE directive End users can send their old devices manufac tured by KNAUER back to the...

Page 43: ...ssurance for laborato ries HPG High Pressure Gradient Operating mode of an HPLC sys tem The solvent is mixed on the high pressure side of the pump HPLC High Performance Liquid Chromatography Solvent Mobile phase eluent or carrier for liquid chromatography Remote External control with chromatography software or analog control signals ...

Page 44: ... see location 12 IQ 31 L LAN port 20 problems 32 router 19 settings 17 setup 18 leak 10 Line voltage 13 Location 12 M Maintenance 34 maintenance contract 35 Modifiers 8 O Operation Arrow keys 22 OQ 31 P port LAN 20 power cable 10 power strip 10 Power supply 13 power supply 10 professional group 9 Pump control 20 Pump head exchanging 35 Installing 36 removing 35 Pumpensteuerung 22 R Remote terminal...

Page 45: ... V6870 version 3 1 Steuerung der Pumpe 22 Switch on 20 T Technical Data 42 Technical data 39 Technical support 12 35 test Installation Qualification 31 Operation Qualification 31 transport damage 41 W Warning instructions 9 warranty 41 ...

Page 46: ...46 Index P 2 1S P 4 1S user manual V6870 version 3 1 ...

Page 47: ...t update 29 08 2016 Printed in Germany on environmentally friendly paper from sustainable forests AZURA is a registered trademark of KNAUER Wissenschaftliche Geräte GmbH www knauer net downloads KNAUER Wissenschaftliche Geräte GmbH Hegauer Weg 38 14163 Berlin Phone 49 30 809727 0 Fax 49 30 8015010 e Mail info knauer net Internet www knauer net HPLC SMB Osmometry KNAUER 2015 V6870 0 01 10 12 Westkr...

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