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iQue

Screener PLUS Hardware Manual: Part Number 11811 Rev B, Maintenance: Manual Control Mode

Manual Control Mode

Overview

Manual Control Mode is a feature of the ForeCyt

software that allows you to interact with the various instrument

subcomponents directly. This can be helpful when troubleshooting instrument problems.

Accessing Manual Control Mode

To access Manual Control Mode click

Device > Manual Control Mode

This will bring up the Manual Control Mode screen.

What follows are some common tasks that Manual Control Mode can help with.

Summary of Contents for iQue Screener PLUS

Page 1: ...iQue ScreenerPLUS hardware manual December 2016...

Page 2: ......

Page 3: ...cautions 11 General Warnings 11 Operator Safety 12 Safety Symbols and Labels 13 Biological Handling Precautions 14 Laser Precautions 14 Operational Precautions 15 Electrical Precautions 15 GETTING TO...

Page 4: ...1 MAINTENANCE 55 Recommended Maintenance Schedule 57 Standard Cleaning Procedures 59 Changing the Probe and Tubing 63 Fluidics Maintenance 69 Managing Plate Models 75 Manual Control Mode 83 Clog Preve...

Page 5: ...otographic mechanical or otherwise for any purpose without the express written permission of IntelliCyt Corporation Trademarks IntelliCyt HTFC iQue ForeCyt Screening Solutions for Life and iDM are tra...

Page 6: ...may accept the Product AS IS or return the Product and receive a refund of fees paid for the Product 6 Limited Warranties Exclusion of Certain Warranties 6 1 Limited Warranty IntelliCyt warrants that...

Page 7: ...r shall not assign any of its rights or obligations under this Agreement without IntelliCyt s prior express written consent which may be granted or withheld at IntelliCyt s sole discretion Any attempt...

Page 8: ...NSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY WHETHER FOR BREACH OF CONTRACT TORT INCLUDING NEGLIGENCE OR OTHERWISE ARISING OUT OF OR RELATED TO THIS AGREEMENT INCLUDING BUT NOT LI...

Page 9: ...5 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Introduction Declaration of Conformity...

Page 10: ...6 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Introduction THIS PAGE INTENTIONALLY LEFT BLANK...

Page 11: ...one quick motion Double click Place the mouse pointer over the item then depress and release the primary mouse button twice in quick succession Drag Place the mouse pointer over the item depress and...

Page 12: ...identify the nature of any potential hazard and determine the actions to perform CAUTION A cautionary statement which if not followed may result in instrument damage or corruption of data Risk of Fire...

Page 13: ...e at 505 345 9075 Opt 2 Before contacting us please be prepared to answer the following questions What were you doing when the problem occurred Did you try to follow the steps in this guide but they d...

Page 14: ...10 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Using This Manual THIS PAGE INTENTIONALLY LEFT BLANK...

Page 15: ...n of the iQue Screener PLUS the system must be connected to an IntelliCyt approved uninterruptable power supply General Warnings THE iQue SCREENER PLUS IS INTENDED FOR GENERAL LABORATORY AND RESEARCH...

Page 16: ...hooting Take full advantage of all safety features and do not defeat safety interlocks and or sensors Acknowledge and act upon instrument alarms and error messages IF THIS INSTRUMENT IS USED IN A MANN...

Page 17: ...threatening Wear appropriate laboratory attire follow universal laboratory safety protocols and adhere to local regulations Electrical Shock Hazard There is the possibility of electrical shock when t...

Page 18: ...or when prompted by software to prevent spillover and possible biohazard risk Consult appropriate Material Safety Data Sheets when necessary The screening system is programmed for automatic decontami...

Page 19: ...including sample tubes and racks Before manually cleaning the system turn off the instrument and unplug the power cord Electrical Precautions Electrical devices pose the risk of an electric shock To...

Page 20: ...16 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Safety THIS PAGE INTENTIONALLY LEFT BLANK...

Page 21: ...must be plugged into an IntelliCyt approved uninterruptable power supply UPS The UPS must be plugged into a standard grounded or earthed mains electrical outlet conforming to local codes Non grounded...

Page 22: ...ticolor Detection BR VBR VYB 6 Simultaneous Color Channels using PMTs 13 Simultaneous Color Channels using PMTs 13 Simultaneous Color Channels using PMTs Label Free Object Detection 2 Light Scatter Ch...

Page 23: ...lasers and six fluorescence channels The table below lists these channels their associated filter and gives a few example dyes for each Channel Position Filter Example Dyes Blue Laser 488nm BL1 530 30...

Page 24: ...le dyes for each Channel Position Filter Example Dyes Violet Laser 405nm VL1 445 45 Pacific Blue BV421 DAPI VL2 530 30 Alexa Fluor 430 H BV510 Qdot525 VL3 572 28 Pacific Orange BV570 Qdot585 VL4 615 2...

Page 25: ...osition Filter Example Dyes Violet Laser 405nm VL1 445 45 Pacific Blue BV421 DAPI VL2 530 30 Alexa Fluor 430 H BV510 Qdot525 VL3 586 20 Pacific Orange BV570 Qdot585 VL4 615 20 BV605 Qdot605 VL5 660 20...

Page 26: ...iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Getting to Know the iQue Screener PLUS 22 Front View Lid Sampling Area Shaker Status Light Light Switch...

Page 27: ...ontroller window will match the physical status light Green Ready Yellow Warning Red Not Ready The iQue Screener PLUS will continue to run if it encounters a warning For example a Waste Bottle is clos...

Page 28: ...umber 11811 Rev B Getting to Know the iQue Screener PLUS 24 Shaker Detail Shaker Deck Gripper Release Lever Gripper Pins 2 of 4 Shaker Body Sampling Area Detail Shaker Cable Guide Microcentrifuge Tube...

Page 29: ...LUS Sampler Detail Contact Sensor Data Cable Sample Tubing Probe Locking Nut Contact Sensing Probe Assembly Probe Guide Z Arm Tubing Pass Through XY Arm Outer Probe Inner Probe Pump Detail Peristaltic...

Page 30: ...811 Rev B Getting to Know the iQue Screener PLUS 26 Right Side Panel Detail Fluidics Station Data Connection Sheath Port Waste Port PLUS One Port PLUS Two Port Bottle Station Sheath Bottle PLUS One Bo...

Page 31: ...11811 Rev B Getting to Know the iQue Screener PLUS Fluidics Harness Detail Fluid Station Data Cable Auto closing Quick Connects Fluid Line Labels Harness Direction Label Back Panel Detail Fuse Holder...

Page 32: ...S 28 Front Service Area Fluidlink Connection Area Sheath Dampener Sheath Filter Sheath Pump CAUTION Fluidlink and sheath filter are the only user serviceable parts in this area In line Sheath Filter S...

Page 33: ...eener PLUS Optical Bench BR Blue Laser Red Laser PMT Module Objective Lens Forward Scatter Detector Flow Cell Focusing Stage 1 of 4 PMT Module Detail BR PMT 1 of 4 Dichroic Mirror 1 of 6 Side Scatter...

Page 34: ...30 Optical Bench VBR Blue Laser Red Laser PMT Module Objective Lens Forward Scatter Detector Flow Cell Violet Laser Focusing Stage 1 of 6 PMT Module Detail VBR PMT 1 of 6 Band Pass Filter 1 of 7 Dich...

Page 35: ...S Optical Bench VYB Yellow Laser Violet Laser PMT Module Objective Lens Forward Scatter Detector Flow Cell Blue Laser Focusing Stage 1 of 4 PMT Module Detail VYB PMT 1 of 6 Dichroic Mirror 1 of 6 Side...

Page 36: ...iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Getting to Know the iQue Screener PLUS 32 THIS PAGE INTENTIONALLY LEFT BLANK...

Page 37: ...Que Screener PLUS Hardware Manual Part Number 11811 Rev B Operation Operation Articles Daily Startup Daily QC Running a Plate Daily Shutdown iQue Screener PLUS Bottle Fluids iQue Screener PLUS Deck Fl...

Page 38: ...34 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A iQue Screener PLUS Operation THIS PAGE INTENTIONALLY LEFT BLANK...

Page 39: ...US Steps 1 Fill the Sheath PLUS ONE and PLUS TWO bottles and empty the Waste bottle Refer to the iQue Screener PLUS Bottle Fluids chapter for detailed instructions 2 Install new deck fluid cartridges...

Page 40: ...tubing clamp are properly installed as shown in the iQue Screener PLUS Startup Wizard and then click Start The system will go through an initialization and startup procedure that takes approximately 3...

Page 41: ...et of Validation Beads and checks their performance against a set of standards For each fluorescence channel the mean fluorescence and CV of the brightest peak is checked 1 Prepare Validation Beads by...

Page 42: ...ics tab and observe the values for the thirteen TEST metrics These are custom calculation metrics that return 1 if the test passes and 0 if the test fails Be sure to scroll through all thirteen test m...

Page 43: ...e p Step 5 Perform Well Identification p Step 6 Analyze your Data Step 1 Prepare a Plate This example experiment was run using a simple suspension cell line Final cell concentration was 1 000 cells pe...

Page 44: ...eriment button on the ForeCyt toolbar In the New Experiment dialog choose Blank Experiment Plate Type 96 Well give your experiment a name and click Save Your new blank 96 well experiment will be creat...

Page 45: ...seconds In the Sample box set the Sip Time to 2 seconds and choose a mapped 96 well plate model that matches the plate you prepared IntelliCyt recommends collecting 1000 2000 events of interest per w...

Page 46: ...r plate on the deck In the Controller Console window click Run As the plate runs you can monitor the run progress by observing the default scatter plot and looking to the Controller Console for additi...

Page 47: ...for Well Identification dialog zoom in choose a gating tool and draw a gate around a population that exists in all of your wells Then click Close At this point your wells are calculated What this mea...

Page 48: ...f the basic techniques in ForeCyt For detailed instructions on ForeCyt data analysis see the video tutorials on the IntelliCyt website A FSC vs SSC scatter plot is used to identify normal and dying ce...

Page 49: ...the iQue Screener PLUS Steps 1 Optional Long Clean At least once a week and particularly after heavy usage perform a Detector Long Clean a Click Clean in the Controller Console b Choose Detector Long...

Page 50: ...46 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Operation Daily Shutdown b Follow the instructions in the Shutdown Wizard and click Start to initiate the shutdown...

Page 51: ...aration SHEATH Hydrodynamic focusing of samples in the flow cell 1 bottle of IntelliCyt Bacteriostatic Con centrate in 1 liter of water Part No 90078 PLUS ONE First phase cleaning solution for cleanin...

Page 52: ...e filtered distilled water 0 22 m filter or equivalent is recommended Filling the Bottles 1 Release the bottle connectors from the tops of the bottles 2 Remove the bottles from the bottle station 3 Op...

Page 53: ...TION Touching the bottle station during a run can trigger a false bottle error thereby stopping the run Do not attempt to fill or otherwise touch the bottles while the instrument is running NOTE For l...

Page 54: ...ill continue to run with warnings but they should be addressed before the next plate is started Do not attempt to address a bottle warning while the system is running Doing so may trigger a false erro...

Page 55: ...This article describes the fluids found in cartridges or troughs in the iQue Screener PLUS sampling area In the sampling area of the iQue Screener PLUS there are slots for four quarter module troughs...

Page 56: ...ing after Decon and Cleaner have been run through the line Part No 90285 single Part No 90289 ten HPLC reagent grade filtered distilled water 0 22 m filter or equivalent QSol Buffer IntelliCyt s stand...

Page 57: ...nstall the cartridge in the appropriate slot Note If there is fluid left over in a cartridge at the end of the day reseal the cartridge with laboratory parafilm and store at 2 8 C for up to a week Usi...

Page 58: ...54 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A iQue Screener PLUS Operation THIS PAGE INTENTIONALLY LEFT BLANK...

Page 59: ...r PLUS Hardware Manual Part Number 11811 Rev A Maintenance Maintenance Articles Recommended Maintenance Schedule Standard Cleaning Procedures Changing the Probe and Tubing Fluidics Maintenance Managin...

Page 60: ...56 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance THIS PAGE INTENTIONALLY LEFT BLANK...

Page 61: ...Comment Change Probe and Tubing The recommended interval for a probe and tubing change is every 20 hours An automatic warning will display when a probe and tubing change is due Detector Long Clean Per...

Page 62: ...58 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance THIS PAGE INTENTIONALLY LEFT BLANK...

Page 63: ...ch one does and when to use it All of the standard cleaning procedures are accessed by clicking the Clean button and using the Clean Wizard Accessing the Clean Wizard To perform any standard cleaning...

Page 64: ...the Probe and Tubing Clean Duration slider The default is a 20 minute clean consisting of five minutes of Decon five minutes of Cleaner and ten minutes of water Detector Quick Clean This clean is used...

Page 65: ...to run Detector Debubble This procedure is used to clear air bubbles from the detector fluidic lines and bottles Use if Daily QC shows irregular scatter after fluidics maintenance and when fluids are...

Page 66: ...62 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance THIS PAGE INTENTIONALLY LEFT BLANK...

Page 67: ...e is due Required Materials Part No Description 91088 iQue Probe Tubing Assy for iQue Screener PLUS 5 Pk 91087 iQue Probe Tubing Assy for iQue Screener PLUS 10 Pk 91086 iQue Probe Tubing Assy for iQue...

Page 68: ...e Probe and Tubing Wizard The Change Probe and Tubing Wizard will guide you through the process of changing the probe and tubing To launch the wizard click Device Change Probe and Tubing This will lau...

Page 69: ...65 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance Changing the Probe and Tubing Wizard Steps 1 Remove Probe and Tubing 2 Prepare Probe and Tubing Assembly...

Page 70: ...ware Manual Part Number 11811 Rev A Maintenance Changing the Probe and Tubing 66 3 Replace Probe and Tubing NOTE When routing the tubing in the pump head take care not to crimp the tubing in the locki...

Page 71: ...g Deck Height When the Change Probe and Tubing Wizard completes the Calibrate Deck Height Wizard will automatically launch It is important to recalibrate the deck height after a probe and tubing chang...

Page 72: ...68 Cleaning the New Tubing When the Deck Height Calibration Wizard finishes you will be prompted to clean the probe and tubing Ensure that the Decon Cleaner and Water troughs cartridges in the fluid s...

Page 73: ...NE Plus TWO and Sheath Bottle Filters 1 90080 In line Sheath Filter 1 91093 PLUS FluidLink tubing connector 5 Pk 91095 iQue Screener PLUS Maintenance Kit 10 pack 10 91092 Plus ONE Plus TWO and Sheath...

Page 74: ...Manual Part Number 11811 Rev A Maintenance Fluidics Maintenance 70 When the wizard launches a list of required items is displayed Make sure you have all the supplies that are needed Wizard Steps 1 Em...

Page 75: ...71 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance Fluidics Maintenance 2 Replace Bottle Filters 3 Replace In line Sheath Filter...

Page 76: ...Probe and Tubing 5 Replace FluidLink NOTE Take care when threading the new FluidLink into the detector inlet Make sure the tubing is flush with the ferrule Make sure the threads are aligned and the Fl...

Page 77: ...er PLUS Hardware Manual Part Number 11811 Rev A Maintenance Fluidics Maintenance Correct inner tubing flush with ferrule Incorrect inner tubing protruding Incorrect inner tubing recessed 6 Replace Pro...

Page 78: ...74 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance 7 Fluidics Check 8 Priming the System...

Page 79: ...Plate Model Offset for that well Fixed Sampling Offset Deck Height The deck height position is calibrated during instrument installation and re checked prior to each plate This is done by having the c...

Page 80: ...re is a field for Plate Model This is where the plate model is selected By default the system comes with only two plate models listed the Generic 96 Well Plate and Generic 384 Well Plate models Additi...

Page 81: ...ging Plate Models Adding a New Plate Model 1 Click Device Manage Plate Models 2 In the Manage Plate Models screen click Add and enter the name manufacturer and type for the new plate model 3 Click Sav...

Page 82: ...78 4 This will launch the Map and Calibrate Wizard Place an example of the plate you are adding in the gripper and click Next As soon as you click Next the instrument will map twelve or more depth po...

Page 83: ...is means that the probe tip will go to a location 5 mm above the bottom of the well This is optimal for most assays However there may be situations where sipping higher or lower in the well is require...

Page 84: ...Set Offset Location step The normal sample position is from the center of the well When using flat bottom plates however the vortexing action of the shaker causes sample to accumulate on the edges of...

Page 85: ...then use the X and Y arrows to move the probe to an offset position No XY Offset probe samples from center XY Offset probe samples from edge of well 96 Well v bottom plate shown of the well 96 well fl...

Page 86: ...n the Edit Plate Model window click Make Default 4 A confirmation prompt will appear Click Yes 5 Click Save in the Edit Plate Model screen The Plate Model is now the default and will be used on new Ex...

Page 87: ...yt software that allows you to interact with the various instrument subcomponents directly This can be helpful when troubleshooting instrument problems Accessing Manual Control Mode To access Manual C...

Page 88: ...well To find a shaker speed that is safe fill a plate with water at the volume you want to test place it on the deck and use the Shaker Control to turn the shaker on to test various speeds Verify Samp...

Page 89: ...isolate these subsystems and troubleshoot 1 Click the Water button to move the probe over the Water cartridge trough 2 Click the Z down button to lower the probe into the water 3 Unplug the tubing fr...

Page 90: ...86 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance THIS PAGE INTENTIONALLY LEFT BLANK...

Page 91: ...bing If the slugs are not moving at all or if they are moving very erratically you may have a partial or total clog Types of Clogs Probe and Tubing Clog The most common clog exists in the probe and tu...

Page 92: ...ween 1 3 x106 cells mL Use of higher cell densities may clog the instrument Certain sample types are prone to aggregation so it may be necessary to include small amounts of an additive such a QSol buf...

Page 93: ...cond Buffer rinse configured as three 1 second rinses In this example we ve created a 3 second rinse after every 6 wells which will add about 50 seconds to a 96 well plate run However it can sometimes...

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Page 95: ...91 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Special Topics Special Topics Articles Moving an iQue Screener PLUS Connecting iQue Screener PLUS Power and Data Cables Purge and Prime...

Page 96: ...92 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Special Topics THIS PAGE INTENTIONALLY LEFT BLANK...

Page 97: ...bottles and troughs 3 Wipe work surfaces down with disinfectant 4 Turn off ForeCyt and shut down the computer 5 Unplug power data cables and fluidics lines Moving the Instrument 1 Due to its weight 4...

Page 98: ...11811 Rev B Special Topics Moving an iQue Screener PLUS 94 After a Move 1 Reconnect the power and data as described in Connecting iQue Screener PLUS Power and Data Cables 2 Fill all the bottles and t...

Page 99: ...also any time that the instrument needs to be physically moved to a new location The iQue Screener PLUS Uninterruptible Power Supply Each iQue Screener PLUS comes with an Uninterruptible Power Supply...

Page 100: ...on the UPS this is not required for safety but having the computer on battery backup can prevent work interruption in the case of a power outage CAUTION The iQue Screener PLUS s UPS is required equipm...

Page 101: ...97 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Special Topics Connecting Power and Data Cables 110V Power and Data Connection Diagram 220V Power and Data Connection Diagram...

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Page 103: ...ove all fluids from the lines This prevents bacterial growth and protects the fittings and lines from freezing Subsequently any iQue Screener PLUS that has been purged needs to be re primed before it...

Page 104: ...al Part Number 11811 Rev B Special Topics Purge and Prime 100 3 Ensure that you have the required supplies and time to complete the operation as shown in the introduction to the Purge Fluidics Wizard...

Page 105: ...101 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Special Topics Purge and Prime 5 Wizard Step 2 Purging System 6 Wizard Step 3 Purge Pressure Sensor Lines...

Page 106: ...iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Special Topics Purge and Prime 102 7 Wizard Step 4 Reconnect Pressure Sensor Lines and Power Down...

Page 107: ...ty 500 mL beaker 1 Ensure that instrument power and data are properly connected as shown in Connecting iQue Screener PLUS Power and Data Cables 2 Ensure that the bottles are filled and the bottle stat...

Page 108: ...iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Special Topics Purge and Prime 104 6 Wizard Step 1 Initial Prime 7 Wizard Step 2 Priming the Pressure Sensors...

Page 109: ...ener PLUS Hardware Manual Part Number 11811 Rev B Special Topics Purge and Prime 8 Wizard Step 3 Reconnect the Pressure Sensor Lines and Install Sheath Filter 9 Wizard Step 4 Connect the FluidLink Pro...

Page 110: ...ner PLUS Hardware Manual Part Number 11811 Rev B Special Topics Purge and Prime 106 10 Wizard Finish Final Prime After the system has been primed click Power on Detector to start the detector see Dail...

Page 111: ...IntelliCyt Technical Support IntelliCyt Corporation 9620 San Mateo NE Albuquerque NM 87113 USA www intellicyt com Telephone 505 345 9075 opt 2 E mail support intellicyt com...

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