InnovaPrep Concentrating Pipette Select User Manual Download Page 9 | Manualshive

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Copyright 2018, InnovaPrep LLC

Patented

https://www.innovaprep.com/patents

9

TABLE 2.1 CONCENTRATING PIPETTE SELECTION GUIDE

Part Number

Filter Tip

Media Type

Pore Size

Membrane

Surface Area

cm

2

Input Sample

Volume (Varies

by Matrix)

Final Concentrated

Sample Volume

(User Selectable)

Processing Rate

(Varies by Matrix)

mL/min

Notes

CC08000-10

Flat Membrane

Polycarbonate

Track Etch

0.4 μm

8.5

Up to 1 Liter*

200 - 1000 μL

Up to

100 mL /min.

Recommended for concentrating bacteria,

whole cells, spores, pollen, and parasites from

food and beverage matrices or samples

containing proteins and polysaccharides

CC08001-10

Flat Membrane

Polyethersulfone

0.1 μm

8.5

Up to 1 Liter*

200 - 1000 μL

Up to

100 mL /min.

Recommended for concentrating bacteria,

whole cells, spores, pollen, and parasites from

environmental samples

CC08018-10

Hollow Fiber

Polysulfone

0.45 μm

98

Up to 5 Liters*

150 - 1000 μL

Up to

150 mL/min

Recommended for concentrating bacteria,

whole cells, spores, pollen, and parasites from

high fouling matrices

CC08022-10

Hollow Fiber

Polysulfone

0.2 μm

98

Up to 5 Liters*

150 - 1000 μL

Up to

150 mL/min

Recommended for concentrating bacteria,

whole cells, spores, pollen, and parasites from

all sample types

CC08020-10

Hollow Fiber

Polysulfone

0.05 μm

98

Up to 3 Liters*

150 - 1000 μL

Up to

90 mL/min

Recommended for virus concentration. The

addition of Tween 20 to the sample is

recommended to aid recovery efficiency (add

10% solution of Tween 20 in a 1/100 ratio to

the sample)

CC08003-10

Hollow Fiber

Polysulfone

Ultra -

Filtration

98

Up to 500 mL*

150 - 1000 μL

50 mL/min with

Tween 20

* With regards to starting sample volume, it is recommended that the entire sample should be processed within 30 minutes. It has been observed that there is a reduction
in the recovery when processing times exceed 30 minutes. The recommended upper-end run volume as shown in the concentrating pipette tip selection guide and time of
30 minutes is a general recommendation and may be dependent on sample matrix and target organism. See Section 10 for full part number list.

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Summary of Contents for Concentrating Pipette Select

Page 1: ...Copyright 2018 InnovaPrep LLC Patented https www innovaprep com patents 1 ...

Page 2: ...STALLING AND REMOVING CONCENTRATING PIPETTE TIPS 15 5 NAVIGATING THE MENU 16 5 1 THE MAIN MENU 16 5 2 THE RUN PROTOCOL MENU 16 5 3 THE MAINTENANCE MENU 17 5 4 THE INSTRUMENT SETTINGS MENU 17 6 MAINTENANCE PROCEDURES 18 6 1 STARTING UP THE INSTRUMENT FOR THE FIRST TIME 18 6 2 THE START UP PROCEDURE 19 6 3 THE SHUT DOWN PROCEDURE 19 6 4 PRIMING THE ELUTION FLUID 20 6 5 PREPARING THE INSTRUMENT FOR T...

Page 3: ...p LLC Patented https www innovaprep com patents 3 9 TIPS AND SUGGESTIONS 30 10 SAFETY PRECAUTIONS 30 11 CONCENTRATING PIPETTE SUPPLIES 32 12 CHEMICAL COMPATIBILITY GUIDE 34 13 CUSTOM PROTOCOL RECORDS 36 14 REVISION HISTORY 37 ...

Page 4: ...ed onto a disposable porous membrane filter with high recovery efficiencies FIGURE 1 1 INNOVAPREP CONCENTRATING PIPETTE SELECT 1 2 APPLICATIONS The patented InnovaPrep concentration technology US Patents 8 110 112 8 584 535 8 758 623 9 593 359 9 574 977 9 738 918 D688 805 and other patents awarded and pending see www innovaprep com patents has application anywhere that enrichment of low concentrat...

Page 5: ...rd and Power Supply North America and 12V Power Supply HC03019 HC03093 Power supply for CP Select See Section 4 1 Power Cord available for North America HC03019 EU HC03208 and UK HC03209 Tubing Clip HC08017 Keeps permeate line secure to waste container Permeate Line HC08006 Directs filtered fluid to waste container See Section 4 2 Alcohol Prep Pads Maintenance Tip 11 µm Nylon Syringe Filter 25mm P...

Page 6: ...ed into the Tip Interface Port during the Start Up Shut Down and Prime maintenance procedures DO NOT USE WITH THE ELUTE OR RUN PROTOCOL DAMAGE CAN OCCUR TO THE INSTRUMENT See Section 6 Alcohol Prep Pads HC08529 Used for periodic cleaning of the tip and elution fluid ports Filterless Tip HC08524 M Used for alternate cleaning and maintenance procedures Also for troubleshooting and diagnostic procedu...

Page 7: ...g through the membrane Thus the CPTs cannot be re used since air trapped inside the housing cannot be pulled through the filter prior to processing a second sample The concentration process is performed in the following steps 1 The main pump draws a vacuum on the Permeate Port The air inside the filter housing is drawn out and the liquid sample is pulled up through the Sample Port 2 As the sample ...

Page 8: ...ble solids As they travel across the surface of the filter they move as a rigid body with a narrow lubricating layer effectively squeegeeing the particles off of the surface Exfoliating Action As the micro bubbles in the foam impact against each other and burst the turbulence and energy produced helps to lift particles that are adhering to the membrane 2 3 FOAM GENERATION The Wet Foam Elution proc...

Page 9: ...ating bacteria whole cells spores pollen and parasites from high fouling matrices CC08022 10 Hollow Fiber Polysulfone 0 2 μm 98 Up to 5 Liters 150 1000 μL Up to 150 mL min Recommended for concentrating bacteria whole cells spores pollen and parasites from all sample types CC08020 10 Hollow Fiber Polysulfone 0 05 μm 98 Up to 3 Liters 150 1000 μL Up to 90 mL min Recommended for virus concentration T...

Page 10: ...e instrument is concentrating and green when the instrument is eluting Tip Interface Ports This is the point of installation for the consumable Concentrating Pipette Tips The foam Elution Port is in front where the foam is introduced into the tip at the time of elution The sample fluid exits the rear Permeate Port during a sample run Concentrating Pipette Tip CPT This consumable component is the o...

Page 11: ...ly stows on the bracket located on the right side of the instrument Canister Interface Port This spring loaded port keeps the proper pressure on the valve of the fluid canister sometimes referred to as Can it contains a check valve which keeps the instrument pressurized when changing canisters The Storage Fluid Adapter also interfaces with this port during the Shut Down Maintenance Protocol Contro...

Page 12: ...nstrument from a computer to download periodic software updates from the manufacturer 12V DC Power Jack Port for the included power supply Power Switch The up position turns the instrument on and the down position turns the instrument off Permeate Line Port This quick connect fitting is where the filtered fluid Permeate exits the instrument This port is valved to prevent fluid from being pumped ou...

Page 13: ...lace Place the open end of the permeate line tubing into a waste container of adequate volume relative to your sample volume To remove the Permeate Line simply press the metal button on the Permeate Port and it will be released 4 3 INSTALLING THE STORAGE FLUID AND FLUID ADAPTER A daily Shut down Maintenance Procedure is described in Section 6 3 The procedure requires the use of the following items...

Page 14: ... canister is removed before it is completely empty a small amount of fluid approximately 100 µL may be expelled from the can this is normal The Canister Interface Port is the first place where contamination can enter the instrument s fluidics Never let dust or dirt enter the port and use good lab practices to prevent bacterial contamination This would include not touching the stem of the Elution F...

Page 15: ...Fluidics Head When the CPT is fully seated you should feel it click into place You can also verify that that the CPT is fully seated by looking through the clear fluidics manifold The rubber O rings in these ports should be occasionally lubricated with the Silicone lubricant item HC08535 20 provided with the instrument to maintain a tight seal With a gloved finger rub a very thin layer of lubrican...

Page 16: ...m ss Name of the selected concentration run protocol defaults to FLAT Start Run Return Selecting the named protocol FLAT will navigate to the Protocol Setup screen where another protocol can be selected created see Section 8 5 Creating and Saving a Concentration Run Protocol or deleted There are three protocol options that have been preprogrammed by the factory named FLAT HOLLOW and ULTRA These ha...

Page 17: ...UMENT SETTINGS MENU There are seven instrument settings A description of each are listed below Beep volume changes the volume of the beeps that occur at the end of concentration cycles and elutions The four options are low med high and off Default low Power LED turns the blue LED light in the Fluidics head on and off Default on Run LED turns the flashing red LED light in the Fluidics head on and o...

Page 18: ...ster Interface Port and prime CP unit a Insert an Elution Fluid Canister into the Canister Interface Port and check for leaks b From the Main Menu select Maintenance Prime and follow the menu prompts Check for production of foam c Remove Elution Fluid Canister d Ensure there is no leakage from the Canister Interface Port e Re install Elution Fluid Canister 3 Check flow rate a From the Run Protocol...

Page 19: ...the required instrument components have been installed Power Supply Section 4 1 Permeate Line Section 4 2 Storage Fluid and Adapter Section 4 3 Step 2 Power instrument on Advance to the Main Menu by pressing Enter on the control panel Step 3 Select Maintenance Start Up Step 4 Follow the menu prompt Place the Maintenance Tip into Tip Interface Port as shown in Figure 6 1 using the same procedure de...

Page 20: ... menu prompt Place Maintenance Tip into Tip Port Select Continue Step 3 Follow the menu prompt Place Foam Can Elution Fluid Canister Into Position Select Continue Fluid from the Canister will rinse through to permeate The Run Protocol menu will be displayed upon completion so use can be resumed 6 5 PREPARING THE INSTRUMENT FOR TRANSPORT OR SHIPMENT It is important to prepare the instrument before ...

Page 21: ...p tightly Warning contents will spill if the unit is shipped with the Wash Solution bottle installed onto the adapter Step 10 At the menu prompt Place Maintenance Tip into Tip Port Select Continue Maintenance Tip is already in place Step 11 Continue past the menu prompt Insert Storage Fluid Line into Can Port Select Continue The canister port must remain open to allow fluid to be flushed out of th...

Page 22: ...eded Available at www Innovaprep com or your local distributor Buildup can occur in the permeate hose after extended use Replace Maintenance Tip HC08005 After Approx 50 Shut Down and or Start Up Routines Available at www Innovaprep com or your local distributor Wear over time will cause Maintenance Tips to fit loosely which may cause leakage or insufficient uptake of the storage fluid Replace Tube...

Page 23: ...ise the Fluidics Head out of the sample container and hold the desired final sample container under the tip of the CPT Select Elute on the screen and press Enter the concentrated sample will be dispensed from the CPT into the final sample container Wait until the progress bar on the screen has filled up before removing the sample vial Additional elutions may be performed by selecting Elute Again A...

Page 24: ...the Permeate Bottle over the barbed end of the quick connect fitting To re install the quick connect fitting first make sure that the metal button on the Permeate Port is pushed down on the instrument Then insert the quick connect fitting on the permeate tubing into the Permeate Port When the fitting is fully seated the metal button will pop up thus locking it in place Replacement Permeate Vent Fi...

Page 25: ...00 µL final volumes are desired For example in some situations recovery may be more efficient if two 100 ms pulses are performed rather than one 200 ms pulse while maintaining the same final elution volume Min 1 ms Max 25 ms 8 2 ADV OPTIONS Complex interactions occur between many of the advanced option settings and as such the advanced options should be used only when absolutely necessary When cha...

Page 26: ...r flow rate CPTs such as the ultrafilter CPTs may require a higher set point Min 1 second Max 60 seconds Ext Delay Sec This sets the delay time between the vacuum relief and the foam valve opening The delay allows the pressure on the permeate side of the membrane in the CPT to achieve equilibrium Changes to the Valve Open time and Pulse count should be considered first Min 0 1 seconds Max 10 0 sec...

Page 27: ...ken to a menu with the Protocol name at the top and standard or advanced settings for the protocol below Standard settings will be shown if Protocol Std has been previously selected in the System Settings Advanced settings will be shown if Protocol Adv has been previously selected in the System Settings This process is described in Section 5 4 The Instrument Settings Menu 8 5 CREATING A CUSTOM PRO...

Page 28: ...enu select FLAT or the last protocol name This will advance you to the Running Protocols menu Step 2 Select Delete Protocol If custom protocols have been created Using the Up arrow and Down arrow you may now go to each available Protocol and then select the desired Protocol for deletion by pressing Enter Select Return to be returned to the Run Protocol menu 8 8 PASSWORD PROTECTION In order to allo...

Page 29: ...ne between 4 and 10 seconds 6 Check Canister Interface Port and prime CP unit a Insert an Elution Fluid Canister into the Canister Interface Port and check for leaks b From the Main Menu select Maintenance Prime and follow the menu prompts Check for production of foam c Remove Elution Fluid Canister d Ensure there is no leakage from the Canister Interface Port e Re install Elution Fluid Canister 7...

Page 30: ...Guide If the instrument has been idle for an extended period the quality of the foam may be reduced due to off gassing of CO2 in the fluid lines The Start Up procedure and Prime functions should be used prior to the first sample run of the day Ensure the Permeate Line fitting is fully seated in its quick connect port on the side of the instrument otherwise the fluid will not flow through the instr...

Page 31: ...s also approved for use on the instrument in the User s Guide see page 33 https uploads ssl webflow com 57aa3257c3e841c509f276e2 5f8626f9e4f54e0ba93d6fc1_CP 20Select 20User 20Guide 2002 2420 compressed pdf For work with BSL2 samples refer to and follow your laboratory s SOPs Wear proper personal protective equipment gloves lab jacket safety glasses or face shield A respirator or mask is recommende...

Page 32: ... rapid analytical methods One canister provides up to 30 elutions Available in single or six pack HC08558 Storage Fluid 30 mL storage fluid bottle for daily shut down protocol Each bottle provides sufficient volume to perform about 10 daily cycles HC08558 5 Storage Fluid 5pk Maintenance Tip Included Five 30 mL storage fluid bottles for daily shut down protocol Each bottle provides sufficient volum...

Page 33: ...r Supply CP Select 12v Power supply for field use HC08535 20 Silicone Lubricant 1cc Tube 20pk HC03173 Elution Port Cap Protects the fluid canister interface port while the Concentrating Pipette is not in use HC08006 Permeate Line HC03093 Power Supply 12V 36W CP HC08005 Maintenance Tip HC03019 AC Power Cord 3 wire ...

Page 34: ...ic acid L L L Acetic acid glacial NR NR NR Acetone NR NR NR Acetonitrile NR U NR 0 1 N Ammonium hydroxide R R R Conc Ammonium hydroxide NR NR NR Amyl acetate NR NR NR Amyl alcohol NR NR NR Aniline NR NR NR Benzene NR NR NR Butyl acetate NR NR NR Butyl alcohol L L L Carbon tetrachloride NR NR NR Chloroform NR NR NR Chromic acid NR NR NR Cresol NR NR NR Cyclohexanone NR NR NR Diacetone alcohol NR NR...

Page 35: ...tate NR NR NR 50 Methyl alcohol NR NR NR 95 Methyl alcohol NR NR NR Methyl chloride NR NR NR Methyl ethyl ketone NR NR NR Methylene chloride NR NR NR Mineral spirits NR NR NR 5 Nitric acid L L L 25 Nitric acid NR NR NR Conc Nitric acid NR NR NR Perchloroethylene NR NR NR 0 5 Phenol NR NR NR 10 Phenol NR NR NR 0 1 N Sodium Hydroxide R R R Conc Sodium Hydroxide NR NR NR Sodium hypochlorite 10 bleach...

Page 36: ... Delay Sec Protocol Name _________________________________________________________________________________ Date ___________________ Created by ___________________________________________________________ Created for ____________________________________________________________________________________ Protocol Name Valve Open ms Pulse Foam Factor Valve Closed ms Flow Start Sec Flow End Sec Flow Min S...

Page 37: ...3377 General Inquiries info innovaprep com Sales sales innovaprep com Customer Service customerservice innovaprep com 14 REVISION HISTORY Revision Date Author Rev 1 September 2017 A Packingham Rev 2 October 2017 A Packingham Rev 3 January 2018 A Packingham Rev 4 March 2018 A Packingham Rev 5 April 2018 D Alburty Rev 6 February 2020 J Birkenholz ...

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