Copyright 2018, InnovaPrep LLC
Patented
https://www.innovaprep.com/patents
23
7
P
ERFORMING A SAMPLE
C
ONCENTRATION
R
UN
Once the instrument is set up, performing and repeating sample concentration runs is straightforward.
Step 1
– Ensure the instrument components have been installed: Power Supply (Section 4.1), Permeate Line
(Section 4.2), and Elution Fluid Canister (Section 4.4).
Step 2
– Power instrument on. Advance to the Main Menu by pressing
Enter
(
⏎
) on the control panel.
Step 3
– Select the CPT of choice (See Table 2.1 Concentrating Pipette Tip Selection Guide) and insert the CPT
into the Tip Interface Port as instructed in Section 4.5).
Step 4
– Check the protocol listed on the Run Protocol screen. Does the listed protocol match the CPT type you
have chosen? If so continue to step 5. If not, review Section 5.2 for the appropriate selection.
Step 5
– Place your sample fluid container on the sample platform and lower the CPT into the sample fluid.
Make sure the tip is all the way to the bottom of the container. Select
Start Run.
The vacuum pump will
immediately start to draw the sample through the CPT. After several seconds, fluid will be seen flowing from the
permeate line into the waste container. Once all of the fluid in the sample container has been drawn through
the CPT, the flow sensor in the fluidics head will detect that the flow has stopped and the instrument will
conclude the run automatically. The menu will display the Run complete screen which shows the processing
time, and gives the option to
Elute
,
Wash
, or
Return
to the Main Menu.
Step 6
– Select
Elute.
Follow the menu prompt:
Position Elution Cup Under Tip.
Raise the Fluidics Head out of
the sample container and hold the desired final sample container under the tip of the CPT. Select Elute on the
screen and press
Enter
(
⏎
), the concentrated sample will be dispensed from the CPT into the final sample
container. Wait until the progress bar on the screen has filled up before removing the sample vial. Additional
elutions may be performed by selecting "Elute Again".
After the desired elutions of the CPT have been performed, remove the CPT and dispose as appropriate for the type of
sample that was processed. Periodically clean the CPT Interface Ports on the underside of the Fluidics Head using an
Alcohol Prep Pad and re - lubricate them with silicone using the Maintenance Tip as explained in Section 4.5.
Alternate Wash Function.
Alternatively, the
Wash
function may be selected to allow the user to perform a wash
step. A wash step can be used to improve the efficiency of the buffer exchange and remove additional material
that may interfere with subsequent analysis.
Alternate Step 6
– Select
Wash.
Follow the menu prompt:
Add Wash Solution.
Raise the Fluidics Head out of the
sample container and replace the container with a new container holding the wash solution, or pour the wash
solution directly into the original sample container. Continue with the Wash step as you would with a normal
concentration run and follow the prompts to perform an Elute step or a second Wash step.
7.1
C
ONTROLLED
C
OLLECTION OF
P
ERMEATE
In cleanroom settings, the Concentrating Pipette user may desire to collect the permeate fluid in an enclosed container
fitted with a vent filter. This eliminates the remote possibility of airborne contamination. InnovaPrep has a Permeate
Bottle with a Vent Filter available for this use (item HC08530). It comes with a 2-port closure consisting of a 0.2 µm vent
