Copyright 2018, InnovaPrep LLC
Patented
https://www.innovaprep.com/patents
25
8
C
ONCENTRATION
R
UN
P
ROTOCOLS
Concentration Run Protocols are defined by a combination of menu parameters including standard and advanced options.
The parameters allow precise optimization of the elution process; its timing, force, volume, etc. A detailed description of
each parameter is described below in Section 8.1 and 8.2. Nearly all concentration runs can be performed by using only
the standard options. In some cases, however, using the advanced options may be useful in the optimization of the
process for certain matrices and certain applications.
It is important to note that complex interactions occur between
many of the advanced option settings and as such the advanced options should be used only when absolutely necessary.
When changes are made to the advanced options significant time may be required to develop a robust new protocol.
8.1
S
TD
.
O
PTIONS
The final volume of your sample is controlled primarily by the elution fluid valve open time. Under “STD Options”
you can precisely control the number of milliseconds (ms) the valve is open, as well as the number of pulses the
valve performs. The longer the open time and the more pulses performed, the larger the elution volume.
Valve Open ms
controls the length of time that the elution valve is open, per pulse, in milliseconds. Increasing
the valve open time will increase the elution volume. Min = 25 ms, Max = 999 ms.
Pulse Count
is the number of cycles that the elution valve will open and close. Multiple pulses are usually used
when larger (>200 µL) final volumes are desired. For example; in some situations, recovery may be more efficient
if two 100-ms pulses are performed rather than one 200-ms pulse while maintaining the same final elution
volume. Min = 1 ms, Max = 25 ms
8.2
A
DV
.
O
PTIONS
Complex interactions occur between many of the advanced option settings and as such the advanced options
should be used only when absolutely necessary. When changes are made to the advanced options significant
time may be required to develop a robust new protocol.
Foam Factor
sets
the release frequency of the foam valve during elution. If set to 10, the foam valve will power
on for 5 ms, then off for 5 ms, repeatedly for the duration of the valve on time. If set to 5, the foam valve will
power on for 2.5 ms, then off for 2.5 ms, repeatedly for the duration of the valve on time. Changes to the foam
factor may result in improved recovery with certain matrices.
Min = 0 ms, Max = 100 ms
Valve Open ms
controls the time that the elution valve is open for each pulse, in milliseconds. The valve open
time provides the most straightforward way to increase or decrease the elution volume. Min = 25 milliseconds,
Max = 999 ms
Valve Close ms
controls the time that the elution valve is closed between each pulse, in milliseconds. If the pulse
count is set to 1, then this setting is irrelevant. Changes to the valve closed time may result in improved recovery
with certain matrices. Min = 100 ms, Max = 999 ms
Pulse Count
is the number of cycles that the elution valve will open and close. Multiple pulses are usually used
when larger (>200 µL) final volumes are desired. For example; in some situations, recovery may be more efficient
if two 100-ms pulses are performed rather than one 200-ms pulse while maintaining the same final elution
volume. Min = 1ms, Max = 25ms
