Consumables
u
70 % EtOH (as needed)
u
95% formamide/1 mM EDTA (15 ml for up to 8 BeadChips, 17 ml for 16, 25 ml for 24)
u
Alconox Powder Detergent (as needed)
u
ATM (2 tubes per 8 BeadChips)
u
PB1 (310 ml for up to 8 BeadChips, 285 ml for 24)
u
RA1 (10 ml for up to 8 BeadChips, 20 ml for 16, 30 ml for 24)
u
STM (2 tubes per 8 BeadChips)
u
TEM (2 tubes per 8 BeadChips)
u
XC1 (2 tubes per 8 BeadChips)
u
XC2 (2 tubes per 8 BeadChips)
u
XC3 (50 ml for up to 8 BeadChips, 100 ml for 16, 150 ml for 24)
u
XC4 (310 ml for up to 8 BeadChips, 285 ml for 24)
About Reagents
u
Make sure that the label of each STM tube indicates the same stain temperature.
u
Decant only the necessary volume of reagent.
u
Use fresh RA1 for each step that requires it. Properly stored RA1 that has not been dispensed for the
previous resuspension step or this extend and stain step is considered fresh.
u
RA1 might form visible precipitate or crystals. Before each use, hold in front of a light and inspect. Invert
several times to redissolve the solution as needed.
u
The XC4 coat is slippery and makes the BeadChips difficult to hold. Self-locking tweezers grip the
BeadChip firmly and help prevent damage.
WARNING
This protocol uses an aliphatic amide that is a probable reproductive toxin. Personal injury can occur through
inhalation, ingestion, skin contact, and eye contact. For more information, consult the material data safety
sheet for this assay at
www.illumina.com/sds
. Dispose of containers and any unused contents in
accordance with the governmental safety standards for your region.
Preparation
1
Prepare the following consumable:
Item
Storage
Instructions
RA1
-25°C to -15°C
Thaw at room temperature.
2
Place tubes in rack in order of use. Dispense all bottled reagents into disposable reservoirs as they are
needed.
Document # 11322427 v03
For Research Use Only. Not for use in diagnostic procedures.
59
Infinium HD Super Assay Reference Guide