Amplify DNA
This step adds the DNA samples to the plates. The samples are denatured and neutralized to prepare them
for amplification.
Consumables
u
MA1 (2 tubes)
u
MA2 (2 tubes)
u
MSM (2 tubes)
u
0.1N NaOH (15 ml)
u
96-well 0.8 ml microplate (midi) (1 plate)
u
DNA plate with 48 or 96 DNA samples (50 ng/μl) (midi or TCY) (1 plate)
u
Cap mats
Preparation
1
Preheat the Illumina Hybridization Oven in the post-amp area to 37°C and allow the temperature to
equilibrate.
2
Prepare the following consumables:
Item
Storage
Instructions
DNA
-25°C to -15°C
Thaw at room temperature. DNA must be 50 ng/µl, resuspended in TE (10 mM Tris,
1mM EDTA).
MA1
Room
temprature
Thaw at room temperature. Invert 10 times to mix, and then pulse centrifuge to
eliminate bubbles and collect reagent at the bottom of the tube.
MA2
-25°C to -15°C
Thaw at room temperature. Invert 10 times to mix, and then pulse centrifuge to
eliminate bubbles and collect reagent at the bottom of the tube.
MSM
-25°C to -15°C
Thaw at room temperature. Invert 10 times to mix, and then pulse centrifuge to
eliminate bubbles and collect reagent at the bottom of the tube.
3
Apply an MSA1 barcode label to a new 0.8 ml microplate (midi).
Procedure
1
If you do not already have a DNA plate, add DNA into either of the following:
u
Midi plate: 20 µl to each DNA well
u
TCY plate: 10 µl to each DNA well
Apply a barcode label to the new DNA plate.
2
Add 20 µl MA1 into the MSA1 plate wells.
3
Transfer 4 µl DNA sample from the DNA plate to the corresponding wells in the MSA1 plate.
4
Add 4 µl 0.1N NaOH into each well of the MSA1 plate.
Refer to the following figure throughout the Make MSA1 process.
Document # 11322427 v03
For Research Use Only. Not for use in diagnostic procedures.
9
Infinium HD Super Assay Reference Guide