8
If excess liquid is not present, add leftover sample from the amplification plate to create a bolus around
the loading port. Do not use RA1, which dilutes the sample.
Excess liquid is desired because it prevents evaporation and the creation of low-intensity areas.
9
Heat-seal any residual sample in the MSA1 plate with foil and store plates at -25°C to -15°C for up to 24
hours. If more than 24 hours, store at -80°C.
Set up BeadChip for Hybridization
1
Load the inserts containing BeadChips into the hybridization chamber.
u
Position the barcode end over the ridges indicated on the chamber.
u
Keep the inserts steady and level.
2
Place the back of the lid onto the chamber, and then slowly lower the front to avoid dislodging the inserts.
3
Close all four clamps so that the lid is secure and sits evenly on the base without any gaps.
Close the clamps in the following order: top-left, bottom-right, top-right, bottom-left.
4
Place the chamber into the preheated Illumina Hybridization Oven so that the top logo faces you.
You can stack up to three chambers per row for a total of six chambers. Make sure that the feet of the
top chamber fit into the indents on the bottom chamber.
Document # 11322427 v03
For Research Use Only. Not for use in diagnostic procedures.
18
Infinium HD Super Assay Reference Guide