NOTE
If you are using Illumina LIMS, you must select Run and select batches before the robot bed map displays
the correct layout for the DNA plates.
4
Remove caps from the MA1, MA2, and MSM tubes, then place the tubes in the robot tube rack
according to the bed map.
5
Pour 15 ml NaOH into a trough. Place trough on robot bed according to bed map.
6
Place DNA and MSA1 plates on robot bed according to bed map.
Figure 4
Robot Setup for Make MSA1
A
MA1 Tube
B
MA2 Tube
C
MSM Tube
D
MSA1 Plate
E
NaOH Trough
F
DNA Plate (MIDI)
7
At the robot PC, select Run.
8
When prompted, enter the barcode of each DNA plate.
The robot bed map is updated with the DNA plate locations.
9
Place the DNA plates on the robot bed according to the bed map and select OK. The robot begins when
the plates are in place.
10 When the robot has completed the run, vortex the sealed MSA1 plate at 1600 rpm for 1 minute.
11 Centrifuge at 280 × g at 22°C for 1 minute.
12 Remove the cap mat, place the MSA1 plate back on the robot bed, and select OK.
13 When the process is complete, select OK.
14 Remove and seal the MSA1 plate with a cap mat.
15 Vortex the sealed MSA1 plate at 1600 rpm for 1 minute.
Document # 11322427 v03
For Research Use Only. Not for use in diagnostic procedures.
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Infinium HD Super Assay Reference Guide