5
Seal the MSA1 plate with the 96-well cap mat.
u
Orient the mat so that A1 on the cap matches A1 on the plate.
u
Make sure that all 96 caps are securely seated in the wells.
6
Vortex the plate at 1600 rpm for 1 minute, and then pulse centrifuge at 280 × g.
7
Incubate at room temperature for 10 minutes.
8
Remove the cap mat and set aside upside down in a safe location.
9
Add 68 µl MA2 into each well of the MSA1 plate.
10 Add 75 µl MSM into each well of the MSA1 plate.
11 Reseal with the cap mat using the original orientation.
12 Vortex at 1600 rpm for 1 minute, and then pulse centrifuge at 280 × g.
NOTE
Perform the remaining protocol steps in the post-amplification area.
Incubate DNA
This step uniformly amplifies the genomic DNA, generating a sufficient quantity of each individual DNA sample
to be used in the Infinium HD Super Assay.
1
Incubate the MSA1 plate in the Illumina Hybridization Oven for 20–24 hours at 37°C.
Fragment DNA
This step enzymatically fragments the DNA. An endpoint fragmentation is used to prevent overfragmentation.
Consumables
u
FMS (2 tubes)
u
Cap mat
Preparation
1
Preheat the heat block with the midi plate insert to 37°C.
2
Prepare the following consumable.
Item
Storage
Instructions
FMS
-25°C to -15°C
Thaw to room temperature. Invert 10 times to mix.
3
Remove the MSA1 plate from the Illumina Hybridization Oven.
Document # 11322427 v03
For Research Use Only. Not for use in diagnostic procedures.
10
Infinium HD Super Assay Reference Guide