INS0081 EN
JAN 2015
REV. I
SuperSnap
High Sensitivity ATP test for use with Hygiena ATP Monitoring Systems
Part No: SUS3000 & SUS3000X (100 tests)
Description/ Intended Use:
SuperSnap is a self-contained, highly sensitive ATP (adenosine
triphosphate) test device for use with Hygiena luminometers. The
system is used for hygiene monitoring, HACCP-related monitoring of
processing equipment, cleaning validation, and other environmental
monitoring such as allergen prevention programs. SuperSnap is
designed to detect very low levels of organic residue with tolerance to
harsh samples that could affect the bioluminescence reaction. As an
allergen prevention tool, SuperSnap can be used to verify efficacy of
cleaning procedures for removal of product residues which may contain
potential allergenic material. SuperSnap
’s enhanced sensitivity allows
detection of product residues down to 1
– 1,000 ppm depending on
product matrices; this is comparable or better than detection capabilities
of specific allergen test methods.
The system works by measuring adenosine triphosphate (ATP), the
universal energy molecule found in all animal, plant, bacterial, yeast,
and mold cells. Product residues from organic matter left on surfaces
contain ATP. Microbial contamination on a surface contains ATP but
typically in smaller amounts. After proper cleaning, all sources of ATP
should be significantly reduced. When a sample is collected and ATP is
brought into contact with the unique liquid stable Luciferase/ Luciferin
reagent in the SuperSnap test device, light is emitted in direct proportion
to the amount of ATP present in the sample. The luminometer measures
generated light and reports results in relative light units (RLU), providing
information on the level of contamination within seconds. The higher the
RLU number, the more ATP present in the sample, and the dirtier the
surface.
Note: SuperSnap is designed to detect invisible/trace amounts of
residue. Overloading the swab with physical matter by swabbing a
visibly dirty surface will inhibit the bioluminescent reaction and produce
inaccurate results.
Directions:
Instructional Video:
Before beginning testing, turn on luminometer. If luminometer has been
programmed with test locations, select appropriate location before
running test.
1.
Allow SuperSnap to equilibrate to room temperature (21
– 25 °C)
before use. Holding swab tube firmly, twist and pull top of swab out
of swab tube. Condensation may be visible on inside of swab tube;
this is normal.
2.
Thoroughly swab a standard 10 x 10 cm (4 x 4 inches) area for a
typical flat surface. Swab tip is pre-moistened for maximum sample
collection. For irregular surfaces, ensure swabbing technique
remains consistent for each test and swab a large enough area to
collect a representative sample.
Important swabbing technique tips:
Do not touch swab or inside of sample device with fingers.
Rotate swab while collecting sample to maximize sample
collection on swab tip.
Apply sufficient pressure to create flex in swab shaft.
Swab in a crisscross pattern vertically, horizontally, and in
both diagonal directions.
Refer to instructional video for demonstration:
3.
After swabbing, replace swab back in swab tube.
4.
To activate device, hold swab tube firmly and use thumb and
forefinger to break Snap-Valve by bending bulb forward and
backward. Squeeze bulb twice, expelling all liquid down swab
shaft.
5.
Bathe swab bud in liquid by shaking
for 5
– 10 seconds. Once
activated, sample must be read in luminometer within 30 seconds.
6.
Holding luminometer upright, insert entire SuperSnap device into
Hygiena luminometer.
7.
Close lid and press “OK” to initiate measurement. Refer to
instrument manual for operating instructions. Results will be
displayed in 15 seconds.
Interpretation of Results:
Hygiena luminometers are preset with Pass & Fail RLU limits of 10 and
30 RLU. These limits are based on industry standards and published
study recommendations. When using default settings, measurements
less than 10 RLU indicate surface is considered clean. Measurements
between 11-29 RLU indicate a warning, surface is not adequately clean.
If measurement is greater than 30 RLU, surface is considered dirty.
Hygiena recommends setting RLU thresholds according to the
standards of your facility. Visit
to view recommended
practices or contact a Hygiena technical representative for guidance.
SuperSnap is highly sensitive and produces more light per ATP
molecule than other Hygiena tests. SuperSnap is approximately 4 times
more sensitive than UltraSnap (Part No. US2020). Using preset limits of
10 & 30 RLU with SuperSnap will increase the standard of cleanliness;
this is recommended when using SuperSnap in an allergen prevention
program. For allergen testing technical documents, refer to the
resources available at
www.hygiena.com.
Calibration Control:
It is advisable to run positive and negative controls according to Good
Laboratory Practices. Hygiena offers the following controls:
(Part # PCD4000) Calibration Control Kit for Hygiena luminometers
(Part # CK25) ATP Positive Control Kit for ATP Test Devices
Storage & Shelf Life:
Store at 2
– 8 °C (36 – 46 °F)
Test devices will tolerate temperature abuse for 4 weeks at room
temperature (< 25 °C)
Store SuperSnap devices out of direct sunlight.
Devices have a 15-month shelf life. Refer to expiration date on
label.
Disposal:
SuperSnap devices are made of 100% recyclable plastic and may be
discarded accordingly.
Safety & Precautions:
Components of SuperSnap do not pose any health risk when used in
accordance with standard laboratory practice and procedures of this
insert.
SuperSnap test devices are for one-time use. Do not reuse.
For further safety instruction, refer to Safety Data Sheet (SDS).
Hygiena Liability:
Hygiena will not be liable to user or others for any loss or damage
whether direct or indirect, incidental or consequential from use of this
device. If this product is proven to be defective, Hygiena’s sole
obligation will be to replace product or at its discretion, refund the
purchase price. Promptly notify Hygiena within 5 days of discovery of
any suspected defect and return product to Hygiena. Please contact
Customer Service for a Returned Goods authorization number.
Contact Information:
If more information is required, please visit us at
www.hygiena.com
or
contact us at:
Hygiena - International
Phone: +44 (0)1923 818821
Email:
Hygiena - Americas
Phone: 805.388.8007
Email:
