![background image](http://html1.mh-extra.com/html/esco/celculture-co2/celculture-co2_user-manual_2426041018.webp)
CelCulture
8
2.3 Preliminary Cleaning
Remove the protective coating on the shelf supports and air duct, if present.
Use a suitable laboratory disinfectant to disinfect all interior surfaces prior to using the product.
Note:
Do not use chlorine based cleaner
See
section 5.1 Cleaning Procedure
for information on cleaning the unit
2.4 Filling the Humidity Pan
For best operation of the incubator, sterilized distilled, de-mineralized or de-ionized water should be used in
the humidity pan.
Chlorinated tap water is not to be used as chlorine can deteriorate the stainless steel. Tap water may also have
a high mineral content, which would produce a build-up of scale in the reservoir. Water should always be
sterilized or treated with a decontaminant, safe for use with stainless steel as well as safe for the product,
prior to being introduced into the humidity pan.
It is recommended to check the water level and refill the humidity pan once a week.
For normal operation, fill up water up to approximately 1000 ml and for decontamination process, fill water
only up to 400 ml. Place the pan directly on the incubator floor to ensure optimum humidity and temperature
response. The pan needs to be inserted completely to the back of the incubator, so that condensed water can
drip back into the pan.
For Suppressed O
2
model: ensure that the sparger is fully immersed in water.
2.5 Filtered Air Exchange
Filtered air exchange is an intentional ‘leak’ in the chamber to reduce the relative humidity to an acceptable
level and to ensure no condensation occurs within the chamber. A small amount of ambient air is being drawn
from outside of the incubator by the re-circulating fan, through a tubing and 1μm filter to prevent
contamination in the chamber. Air is being ‘pushed out’ through another tubing and 1μm filter installed on the
access port plug located at the back of incubator. Tubing installed inside the chamber for filtered air exchange
is silicon tubing which can withstand high decontamination temperature.
If high humidity level is required, the filtered air exchange can be removed and “Elevated Humidity” can be
activated in the control panel. (Call Esco or your distributor to activate this function)
2.6 First Run
After the incubator has been properly installed, and connected to the power supply, humidity pan filled, and
the unit connected to gas supply, follow the procedures for the unit’s initial start-up.
1.
Switch ON the unit. A welcome message will appear on the display. Press SET to continue.
2.
Keypad operation will appear on the display. Press SET to continue.
3.
Set the date, time, temperature set point, %CO2 set point and %O2 set point:
a.
Set Date. The digit will blink. Use UP/DOWN buttons to select the current date and press SET
to confirm each digit.
b.
Set Time. The digit will blink. Use UP/DOWN buttons to select the current time and press SET
to confirm each digit.
c.
Set Temperature Set Point. Use UP/DOWN buttons to select the temperature set point. Press
SET to confirm
d.
Set %CO2 Set Point. Use UP/DOWN buttons to select the %CO2 set point. Press SET to
confirm.
Summary of Contents for CelCulture CO2
Page 8: ...CelCulture vi...
Page 10: ...CelCulture viii...
Page 14: ...CelCulture 4...
Page 20: ...CelCulture 10...
Page 30: ...CelCulture 20...
Page 38: ...CelCulture 28...
Page 39: ...APPENDIX...
Page 40: ......
Page 42: ......