Esco CelCulture CO2 User Manual Download Page 1 | Manualshive

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Esco CelCulture CO2 User Manual Download Page 1

CelCulture

User
Manual

Thank you for purchasing this Esco CO

2

Incubator. Please read

this manual thoroughly to familiarize yourself with the many
unique features and exciting innovations we have built into
your new equipment. Esco provides many other resources at
our website, www.escoglobal.com, to complement this manual
and help you enjoy many years of productive and safe use of
your Esco products.

Esco Technologies, Inc.
2940 Turnpike Drive, Units 15-16  •  Hatboro, PA 19040, USA
Toll-Free USA and Canada 888-375-ESCO
Tel 215-441-9661  •  Fax 215-441-9660
us.escoglobal.com •  [email protected]

Esco Micro Pte. Ltd.
21 Changi South Street 1  •  Singapore 486 777
Tel +65 6542 0833 •  Fax +65 6542 6920
www.escoglobal.com •  [email protected]

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Summary of Contents for CelCulture CO2

Page 1: ...at our website www escoglobal com to complement this manual and help you enjoy many years of productive and safe use of your Esco products Esco Technologies Inc 2940 Turnpike Drive Units 15 16 Hatbor...

Page 2: ...f such change Celculture is a registered trademark of Esco Material in this manual is provided for informational purposes only The contents and the product described in this manual including any appen...

Page 3: ...ts 6 2 2 Installation 6 2 2 1 Unit Leveling 6 2 2 2 Unit Stacking 5 2 2 3 ULPA Filter Installation 7 2 2 4 Shelves System Installation 7 2 2 5 Connecting the Unit to Electrical Power 7 2 2 6 Connectin...

Page 4: ...al Inspection 21 4 1 5 Calibration of Temperature CO2 O2 and Humidity 21 4 1 6 Change ULPA Filter 21 4 1 7 Change Inline Filter 22 4 2 Maintenance Service Log 23 Chapter 5 Cleaning and Decontamination...

Page 5: ...as filters HEPA ULPA carbon pre filters and fluorescent UV bulbs Esco is not liable for any damage incurred on the objects used on or stored in Esco equipment If the objects are highly valuable user...

Page 6: ...Opti s 5 years for Ascent Max s Cleanroom Equipment 1 year limited Laboratory Ovens and Incubators 1 year limited CO2 Incubators 2 years limited Containment Pharma Products 2 years limited Ultralow Te...

Page 7: ...hly understand the installation procedures and take note of the environmental electrical requirements In this manual important safety related points will be marked with the symbol If the equipment is...

Page 8: ...CelCulture vi...

Page 9: ...ltage Directive and its amending directives 92 31 EEC The Electromagnetic Compatibility Directive and its amending directives has been designed to comply with the requirement of the following Harmoniz...

Page 10: ...CelCulture viii...

Page 11: ...y for culturing mammalian cells Mammalian cells have very stringent requirements of the environment which can be closely monitored and maintained by the use of CO2 Incubators Other applications such a...

Page 12: ...switch 3 Circulation fan 4 ULPA filter 5 Shelves 6 Sampling port 7 Glass door gasket 8 Humidity water pan 9 Leveling feet 10 Control panel 11 Outer door 12 Glass door 13 Pilaster shelf support 14 Gla...

Page 13: ...er inlet 3 RS485 communication 4 Analog output optional 5 Alarm contacts 6 CO2 gas inlet 7 CO2 gas inlet CO2 backup system 8 N2 gas inlet for suppressed O2 control 9 N2 gas inlet for suppressed O2 con...

Page 14: ...CelCulture 4...

Page 15: ...moving the equipment do not lift using the door or door handle The spacing to the side wall should be at least 10 cm 3 9 The spacing at the back of the equipment should be at least 11 cm 4 3 2 1 2 En...

Page 16: ...d to level the unit Adjust the feet until the unit sits level left to right and front to back 2 2 2 Unit Stacking If equipment is to be stacked on top of each other the equipment with the lower workin...

Page 17: ...minants in the CO2 gas supply Check all fittings for leaks Connect one end of the tubing to the barb fitting at the rear of the cabinet labeled CO2 Inlet 1 and install the clamp 2 to properly secure t...

Page 18: ...leak in the chamber to reduce the relative humidity to an acceptable level and to ensure no condensation occurs within the chamber A small amount of ambient air is being drawn from outside of the inc...

Page 19: ...l which prevents the chamber from being contaminated and the chamber environment from being disturbed When the main door is opened the blower fan and gas supply is automatically turned off Note Make s...

Page 20: ...CelCulture 10...

Page 21: ...alue MUTE Button ALARM LED MUTE button will mute the audible alarm for a period of time The red ALARM LED will light up whenever an alarm condition is triggered HEAT LED HEAT LED will light up wheneve...

Page 22: ...AY O2 ALARM RELAY SET ULPA REMINDER REPLACE ULPA FILTER SET LANGUAGE SET DATE SET TIME SET RS 485 ADDRESS RESET DEFAULTS SHOW TEMP DATA LOG SHOW CO2 DATA LOG SHOW O2 DATA LOG SHOW RH DATA LOG DATA LOG...

Page 23: ...ENU ADMIN OVER TEMP ALARM SP 1 X 65 3 2 3 2 Low Temperature Alarm The unit will display sound the alarm when the temperature goes below the low temperature alarm value The default low temperature alar...

Page 24: ...ter audible alarm is disabled MENU ADMIN AUDIBLE ALARM ENABLE DISABLE 3 2 3 11 Alarm Ring Back Time When the system encounter error it will sound the audible alarm the user can temporarily press MUTE...

Page 25: ...ET DATE YYYY MM DD 3 2 3 18 Set Time User can set the time and it will be maintained even after the unit is turned off The format is HHMMSS MENU ADMIN SET TIME HHMMSS 3 2 3 19 Set RS 485 Address for u...

Page 26: ...section 3 3 2 Analog Output Calibration for the instruction 3 2 6 Help This menu section will show brief explanation on the basic control of the unit 3 3 Calibration 3 3 1 Unit Calibration After the...

Page 27: ...r DC 0 5 V or current 4 20 mA mode The factory default setting is voltage The voltage of the analog output can be calibrated using a calibrated digital multi meter To calibrate Rear Panel Terminal MEN...

Page 28: ...RDCY Percentage of average duty cycle of the main heater work over an hour BASE HTRDCY Percentage of average duty cycle of the base heater work over an hour DOOR HTRDCY Percentage of average duty cycl...

Page 29: ...o report RH as read Low temperature Temp reading plus calibration offset is lower than low temp setting 15 minutes Display continues to report temp as read Low CO2 level CO2 reading plus calibration o...

Page 30: ...CelCulture 20...

Page 31: ...there is sufficient water in the pan It is recommended to check the water level and refill the humidity pan with distilled sterile water once a week 4 1 3 Cleaning and Decontamination See chapter 5 Cl...

Page 32: ...CelCulture 22 4 2 Maintenance Service Log It is good practice and in some cases regulatory requirement to maintain a log of all maintenance work carried out on your incubator...

Page 33: ...e When cleaning and disinfecting always observe the safety instructions and hygiene regulations 5 Re assemble the unit by reversing step 3 5 2 Decontamination Decontamination in CelCulture uses 90 C m...

Page 34: ...r are located behind the blower in the top duct To remove the TC or O2 sensor 1 Unscrew all 4 screws at the corners of the sensor assembly 1 2 Lower down the sensor assembly 3 Disconnect the sensor co...

Page 35: ...2 CO2 level is below or above CO2 set point Possible Cause Corrective Actions Unit is not connected to the pressure regulated CO source Check gas hose connection at the rear of unit to the CO2 source...

Page 36: ...idity pan should be half filled Problem 5 Unusually high gas consumption Possible Cause Corrective Actions Air leakage through Inner door gasket Check installation of the gasket ensure it is properly...

Page 37: ...tube is connected to the hole in the blower intake above where the ULPA filter mount Check also 2nd filter is not saturated and tubing is not blocked Loosen the 2 thumbscrews at the front of the top d...

Page 38: ...CelCulture 28...

Page 39: ...APPENDIX...

Page 40: ......

Page 41: ...n introduced to the incubator during its operation problems encountered etc 2 Please also record any major maintenance procedure performed by the service technician for example parts replacement recer...

Page 42: ......

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