ELite 3, User Manual
Page 11/72
1.4.
Parts of the Analyzer
The hematology analyzer is composed of three main units:
Fluidic System:
Performs sampling, diluting, mixing, and lysing
functions. Generates the regulated vacuum used for
moving cells through the aperture during the
counting process.
Data Processing System: Counts, measures and calculates blood cell
parameters, generates and stores numerical results
and histograms.
Control Panel:
Features an LCD display, touch screen, START
button, status LED, and USB port interfaces.
1.5.
The measurement process
For the Schematics of the fluidics system, see Section 13.
Sample aspiration and dilution:
Stages of the blood testing process
a.
25 µl of anti-coagulated (K3-EDTA) whole blood sample is aspirated into the
sampling needle, and mixed with 4 ml of diluent and stored in the chamber
(MIX dilution).
b.
25 µl of the MIX dilution is aspirated into and stored in the needle during WBC
measurement and hemoglobin analysis
c.
Lysing reagent is added to the mix dilution held in the WBC chamber for
WBC differential analysis. This amount of lysing reagent is patient type
dependent and the operator can change it.
d.
After WBC counting, HGB reading and washing process, 4 ml of diluent is
added to the second dilution (using the 25
μ
l of mix dilution stored in the
needle).
e.
This portion is analyzed for RBC count, PLT count and their parameters.
f.
Another washing process prepares the unit for the next analysis.
Table 1.
Dilution rates used:
Measurement times:
MIX dilution
1:160
WBC count
12 seconds
RBC dilution
1:32 000
HGB measurement
3 seconds
WBC dilution 1:196
(depends on lyse amount)
RBC/PLT count
12 seconds