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4. maintenance and problems solving
4.1 instrument maintenance
The instrument should be stored in dry and ventilated condition, avoid direct exposed
to sunlight and dark humid circumstance.
Clean the prick hole of the block, for fear the residual affect the accuracy of the
experiment. Corrosive impregnant is prohibited. It is advised to use cotton stick dipped
with absolute alcohol, and use an air gun to clean each hole.
The dust and waste on the shell should be cleaned with cloth dipped with some clean
water, never use impregnant, alcohol, strong acid or strong alkaline in order not to
damage the exterior.
After completing the experiment, check the instrument, turn off all the switches, clean
the residual the experiment left, including water, the reaction liquid and impurity. Keep the
instrument clean and dry. If don’t use it for a long time, cover the instrument to protect it.
4.2 common problems analysis and corresponding solving method
4.2.1 bad experiment results
If get bad experiment results using this instrument, there may be following
problems:(only for reference):
A. Reaction component is not right or low-quality or small amount of
single-strand area is incorrect.
B.Melting temperature too high or too low
，
refer to reaction volumes,increase or
decrease time.
C. Annealing temperature too high or too low.
D. Reaction component concerntration too high or too low.
E. The preparation haven't disposed especially.
F. Program contains a wrong value.
G. Temperature of the sample or sample electrode is low,while block temperature
is high.
H. Check the recation tube have been placed correctly
，
overlaid the reaction with
mineral oil to enhance heat exchange.
I. After a long time usage, the temperature may loss accuracy, and this could be
adjusted by the producer, customers should never do it by themselves.
