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www.drawell.com.cn

K960 GRADIENT

THERMAL CYCLER

OPERATING MANUAL

Drawell International Technology Limited

Chongqing Drawell Instrument CO,.Ltd

Add:Suite 2705,Building No.12,Shiyou Road No.1,Yuzhong District,

Chongqing,China

Tel: 0086-023-63268643

Shanghai Drawell Scientific Instrument Co.,Ltd

Add : Suite 1117,Lane561 XiuChuan Rd.,PuDong New

Area,Shanghai,China

Web : www.drawell.com.cn

Email : [email protected]

Summary of Contents for K960-A

Page 1: ...ed Chongqing Drawell Instrument CO Ltd Add Suite 2705 Building No 12 Shiyou Road No 1 Yuzhong District Chongqing China Tel 0086 023 63268643 Shanghai Drawell Scientific Instrument Co Ltd Add Suite 1117 Lane561 XiuChuan Rd PuDong New Area Shanghai China Web www drawell com cn Email sales05 drawell com cn ...

Page 2: ... 14 3 1 1 New 14 3 1 2 Edit 16 3 1 3 Copy 16 3 1 4 Delete 16 3 2 Parameter setting 17 3 2 1 Operating parameter setting 17 3 2 2 heat lid setting 17 3 2 3 System setting 18 3 3 Information inquiry 18 3 3 1 Log inquiry 18 3 3 2 Gradient inquiry 19 3 3 3 Help information 19 4 Common Failure Analysis 21 4 1 Failure code and corresponding phenomenon 21 4 2 Common failure analysis and corresponding sol...

Page 3: ...blets of spare fuse AC 250V 10A 9 Other scheduled components If you find the components in the box are not consistent with the list please contact us as soon as possible and our company will try our best to solve the problem Note When you receive the K960 and find the package seriously impaired or the instrument impaired please keep the package and contact us immediately we will replace the produc...

Page 4: ...of the body for fear of the body burn during the whole operation Don t put anything around the instrument closer than 30 cm when it is working to ensure the heat dispersion 1 2 2 Maintenance safety The instrument need timely clean the block and the heat lid to keep the experiment precise It is advised that you take out the temperature changing block before you clean Use a piece of clean soft cloth...

Page 5: ...y innovation and landmark in the field of biological medicine PCR has been widely applied in immunology human genome project forensics oncology population biology paleontology zoology botany research field and clinical diagnosis of virus tumor hereditary disease etc by PCR detection As the gradient PCR becoming the optimal method of optimizing the reaction condition Jingle gradient has become the ...

Page 6: ...xperiment sample Running in a low temperature is the basic feature of the advanced gene amplification instrument Its ability to deal with the sample in low temperature together with the PCR itself make it possible that all procedure can be done automatically This system uses a advanced and reliable semi conductor refrigeration scheme providing an efficient temperature adjusting speed and super wid...

Page 7: ...on hole 2 bottom 3 eet cushion 4 air in hole 5 upper cover 6 button panel 7 display 8 button 9 switch 10 power supply socket 11 Fuse socket 12 network interface 13 USB interface 14 232 interface 15 shell 16 air out hole 17 LOGO 18 heat lid spanner 19 grade knob 20 grade calibration ...

Page 8: ...ucture such as unreliable instability and short using time stay at any angle according to your demand As the above figure shows when you draw the spanner the upper cover come away from the shell and could be fixed at any angle The personalize structure makes the product more beautiful and more convenient to use for the operator Note The angle between the upper cover and the shell range from 0 to 9...

Page 9: ...tions 0 5 0 2 0 2and384 on the panel correspondingly suits the 0 5ml 0 2ml dome 0 2ml flat and 384well test tube Suppose you have to use the 0 2ml flat test tube you just need to take on the block assembly with the 0 2ml groove see details in 3 4 substitutable block assembly then revolve the knob to the calibration of 0 2 flat We use a rational and practical design slam the door of traditional ide...

Page 10: ...le method and the same installment method different block assembly corresponds to different test tubes this kind of design assures the operator can quickly and efficiently replace the block assembly according to their demand handle Figure 1 Figure 1shows the state of replacing the block assembly When the operator is replacing the block pull the holder upward when the holder become vertical the blo...

Page 11: ...www drawell com cn Pull up the handle Take out the block Select the module you need and put it into the shell Replacement steps of module components ...

Page 12: ...k and the shell press the handle down let the alnico on the handle and the shell attract each other Fifth step when the alnico attracts closely the handle will be parallel to the shell and the block will be self locked to the shell The replacement is completed The portable consolidated designed together with the intellectual block self lock structure enables the operator to replace the block more ...

Page 13: ...se and capitalization and open the gradient inquiry table Run Running button when select and open a file press to run the file Switch between minus and plus when programming F1 F2 F3 F4 Function button have different function in different menu windows such as back create delete save etc you can operate it according to the tips Esc Cancel button used to escape from submenus clear the dialog box and...

Page 14: ...the instrument and harm the operator s safety and life 2 2 5 3 instrument performance indicator Model K960 A K960 B K960 C K960 D Sample size 96 0 2mL A 54 0 5mL B 96 0 2mL 77 0 5mL C 384well D Temperature range 0 99 9 Rt 30 Temperature ascending speed 3 s Temperature descending speed 2 8 s Temperature uniformity 0 4 Temperature accuracy 0 1 Gradient temperature range 30 99 Gradient temperature wi...

Page 15: ...segment number setting Press SHIFT button to set the units of the file number of segment units and the times of cycle After selecting the item please ENTER to enter the value using the number buttons If you want to set the extended functions please press Tab to move the cursor to the extended function section press and to move the cursor to select the enable or disable of temperature gradient incr...

Page 16: ...e cursor stay at the stage which has been set grads Press ESC to exit 3 1 1 4 the file name could only be composed of by numbers and characters It can be entirely numbers entirely characters and combinations of numbers and characters You can set the file name by input the numbers or characters in the file name display section Press Tab to change between the capitalization and the lowercase press S...

Page 17: ...ursor to select the file you want to copy 3 1 3 3 press F4 after selecting the system will display a confirm box press ENTER to affirm the copy and press ESC to cancel The system will get into the window of file name setting window and press F4 to save after setting the copied file name 3 1 4 delete 3 1 4 1 move the cursor or press number 1 in the main menu window to select the file submenu press ...

Page 18: ...orage range 0 40 Note Recommendation Temperature lower than 15 is winter mode 15 25 is spring autumn mode higher than 25 is summer mode The mode setting above just for reference only Specific season mode should be setting based on the actual situation of your area in order to improve the accuracy of experimental results 3 2 1 3 Press ESC to exit and the system will show a to save or not dialogue b...

Page 19: ... parameter setting window 3 2 3 2 in the system parameter setting window press SHIFT button to select options such as system date and time hint beep the taxis manner of file list press ENTER to enter the option and input value using the number buttons 3 2 3 3 press ESC to exit and the system will show a to save or not dialogue box press ENTERto affirm and ESC otherwise 3 3 information inquiry 3 3 ...

Page 20: ...ow press and to select the option of center temperature and the gradient range input the value using the numberbuttons The center temperature range 31 99 The gradient width 1 15 The algebra sum of the center temperature and the gradient scale 30 100 Operator can view the temperature of 12 corresponding block rows 3 3 2 3 In the cycle parameter window building or editing a program Press Tab to turn...

Page 21: ...enter the help window You can see connecting information of our company 3 4 others 3 4 1 when the operator enters value out of the permitted scale the system will value it with default value and the input value will not be adopted 3 4 2 the communication submenu in the main menu window is obligated for update and is not valid in this version ...

Page 22: ...rument clean and dry If don t use it for a long time cover the instrument to protect it 4 2 common problems analysis and corresponding solving method 4 2 1 bad experiment results If get bad experiment results using this instrument there may be following problems only for reference A Reaction component is not right or low quality or small amount of single strand area is incorrect B Melting temperat...

Page 23: ...or sensor 6 Short circuit in radiator sensor Contact distributor or manufacture 3 Cooling speed slows down obviously or cannot cooling Ventilation jam Clear the jam Cooling block failure Contact distributor or manufacture Fan failed or stopped 4 Heating speed slows down obviously or cannot heating Cooling block failure 5 Heating and cooling terminated Bad sensor Contact distributor or manufacture ...

Page 24: ...tion of the instrument which proves to be defective as described herein The buyer is responsible for freight to the maintenance shop designated by our company on all warranty claims The buyer is also responsible for the transportation expenses of the freight to the maintenance shop Our company shall be responsible for the transportation expenses of the freight to the buyer After the warranty comes...

Page 25: ...e middle row of the sample base The temperature presented on the right and left side is descending or ascending You can refer to the specific corresponding figure on the grads temperature table This function can significantly increase the efficiency of temperature controlling when the experiment is still in the stage of exploring The ascending and descending of temperature means when another repet...

Page 26: ...e enter the Communication function A This function is still under updating You can only use it when the updating process has been finished 6 5 Q How to set the 4 store function Should we add a section of program at the end of the program A It s totally the wrong way to add a section of program at the end of the program If you do it this way an unwanted result may be produced The right operation is...

Page 27: ...riting program the first 00 represents minutes and the latter means 30 seconds 30 seconds 1 hour and 7 minutes 6 7 Q Can we fix the test tube when we turn the holid button to the corresponding location A for most universal test tubes we only have to turn the button to the location marked on the panel But for some particular or nonstandard test tube we have to turn the button to the extent that we ...

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