Abacus junior 5
User’s Manual
9
1.4 Parts of the Analyzer
Abacus junior 5
hematology analyzer can be divided into three main parts:
Fluidic System:
Performs sampling, diluting, mixing, and lysing
functions. Generates regulated vacuum used for
moving cells through the aperture during the
counting process.
Data Processing System:
Counts, measures and calculates blood
parameters, generates and stores numerical results
and histograms.
User interface/peripherals:
Features an LCD display, a 29-button keypad, and
parallel (external printer) and serial (computer)
interfaces.
1.4.1 Function of the Fluidics
For the Schematics of the fluidics system, see Section 9.
Sample aspiration and dilution:
Sample
processing
procedure
a. Instrument takes sample by piercing the closed tube if necessary, and
aspirates 90µl of blood into the sampling needle.
b. Partial blood samples are taken from this 90µl for each measurement to be
performed later on during the process.
c. The separated volumes are put into different chambers, to the EOS, BAS and
MIX chambers with the necessary amount of the respective reagents to make
the required dilution rates, in incubated conditions.
d. EOS and BAS measurements are made in the WBC chamber, after thorough
cleaning.
e. MIX solution (in MIX chamber) is the basis for the second dilution of RBC, and
for the WBC measurement.
f. Solution from MIX is drawn over to WBC chamber, while LYSE is added to the
blood. Lysing takes place and measurement is performed in WBC chamber.
g. RBC and PLT measurement is performed in RBC chamber.
h. The system is cleaned and washed with CLEANER and DIL solutions.
Table 1.
Dilution rates in
Abacus junior 5
:
Primary dilution
1:160
RBC dilution
1:32 000
WBC dilution
1:200
EOS, BAS dilution 1:160
Measurement time:
WBC / EOS / BAS counts 5 seconds
HGB measurement
2 seconds
RBC/PLT count
8 seconds
