CooperSurgical K-Systems L100 IVF Series, Instruction Manual

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INSTRUCTION MANUAL FOR L100 SERIES WORKSTATIONS
5.1.5 Gassing Station
Most of the culture media that is used in assisted reproduction
techniques is sensitive to changes in pH, it is important to flush the
media with CO
2
.
The pH of the media in the test tubes and dishes may be controlled
by flushing with appropriate gas mixtures.
The pre-mixed CO
2
gas supply is connected to the back of the IVF
Workstation. It is being heated and humidified by the gas washing
bottles placed in the inner wall of the IVF workstation and coming out
through the gassing nozzles built into the table plate. An incubator
hood should be placed above the nozzles at all times.
The flow rate can be adjusted as well as read at the display
in the inner wall
For optimal use, only distilled water should be used.
5.1.6 Gas Mixture
Follow indications from the culture media provider for correct handling of media. Culture media
must be maintained at a pH of about 7.4, this can be obtained by gassing with an atmosphere
of 5% CO
2
.
The most frequently used gas mixtures are 5% CO
2
-in-air, and 5% CO
2
, 5% O
2
and 90% N
2
.