3
6.1.2
Using a Factor ................................................................................................................... 22
6.1.3
Using a Standard ............................................................................................................... 23
6.1.4
Selecting Concentration Units ........................................................................................... 23
6.2
Calibration .............................................................................................................................. 23
6.2.1
Calibrating to a Factor....................................................................................................... 24
6.2.2
Calibrating to a Standard .................................................................................................. 24
6.3
Sample Measurement .............................................................................................................. 25
6.3.1
Measuring a Sample After Calibrating to a Factor.............................................................. 25
6.3.2
Measuring a Sample After Calibrating to a Standard ......................................................... 25
Section 7 - Spectrum .................................................................................................... 26
7.1
Method Set up ........................................................................................................................ 26
7.1.1
Setting Start and End Wavelengths ................................................................................... 26
7.1.2
Setting the Scan Interval.................................................................................................... 27
7.1.3
Selecting Absorbance or % Transmittance ......................................................................... 27
7.2
Calibration .............................................................................................................................. 27
7.3
Sample Measurement .............................................................................................................. 27
7.4
Data Analysis ........................................................................................................................... 28
7.4.1
Peaks and Valleys .............................................................................................................. 28
7.4.2
Area Under Curve ............................................................................................................. 29
7.4.2.1
Area Under Curve - Baseline Mode ............................................................................. 29
7.4.2.2
Area Under Curve - Tangent Mode ............................................................................. 30
Section 8 - Quantitation .............................................................................................. 31
8.1
Method Set up ........................................................................................................................ 31
8.1.1
Selecting a Wavelength ..................................................................................................... 31
8.1.2
Selecting Number of Replicates ......................................................................................... 32
8.1.3
Selecting Concentration Units ........................................................................................... 32
8.2
Measuring Calibration Standards ............................................................................................. 33
8.3
Standard Curve ....................................................................................................................... 34
8.4
Sample Measurement .............................................................................................................. 35
Section 9 - Kinetics ....................................................................................................... 36
9.1
Method Set up ........................................................................................................................ 36
9.1.1
Selecting a Wavelength ..................................................................................................... 36
9.1.2
Setting the Kinetics Measurement Time............................................................................. 37
9.1.3
Setting the Measurement Time Interval ............................................................................. 37
9.1.4
Setting Lag Time ............................................................................................................... 37
9.1.5
Selecting Absorbance or % Transmittance ......................................................................... 37
9.1.6
End Point Concentration ................................................................................................... 37
9.2
Calibration .............................................................................................................................. 38
