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Touch
to save the absorbance results for the standard.
Touch
to add another standard and enter the concentration value for that standard. This
time a blank is not required so the Standard icon is active straight away. Place the known standard solution into
the sample chamber and close the instrument lid. Touch the
icon and the instrument will measure the
absorbance of the standard sample. If there are 2 or more replicates you will be asked to measure replicate 1
followed by the remaining replicates.
Touch
to save the absorbance results for the standard.
Repeat the above process for the number of standards required to create the calibration curve.
A calibration curve can be set up in advance and concentrations saved for future use without measuring the
absorbance values. When the quantitation assay is next performed, each standard is read to calculate the standard
curve. To activate this turn
to on. This can aid in the preparation of frequently used
quantitation assays.
8.3
Standard Curve
Following the measurement of each standard the calibration curve is displayed.
