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Introduction ____________________________________________________________________ 2
General safety instructions _______________________________________________________ 3

Dangers associated with the operation  __________________________________________________________ 3
Photobiological safety LED, important safety instructions  __________________________________________ 3
Prevention of biological and infectious hazards  ___________________________________________________ 3
Disinfection and decontamination ______________________________________________________________ 4
Environment, storage and use __________________________________________________________________ 5

Models _________________________________________________________________________ 6
Objectives ______________________________________________________________________ 6
Components of the microscope ___________________________________________________ 7
Preparing the microscope for use __________________________________________________ 8

Assembling steps _____________________________________________________________________________ 8

Operation ______________________________________________________________________ 9

Setting up the illumination _____________________________________________________________________ 9
Placing the specimen slide  _____________________________________________________________________ 9
Focusing and slide protection _________________________________________________________________ 10
Adjusting the focusing tension  ________________________________________________________________ 10
Eyepieces ___________________________________________________________________________________ 10
Abbe condenser  _____________________________________________________________________________ 11
The field (Köhler) diaphragm __________________________________________________________________ 11
Adjusting the aperture diaphragm _____________________________________________________________ 11
Use of the S100x oil-immersion objective ________________________________________________________ 12
Illumination _________________________________________________________________________________ 12

Phase contrast (model 78904-10) ______________________________________________ 12-13
Supplementary manual for fluorescence attachment (model 78904-13) ____________ 14-16
Maintenance and cleaning _______________________________________________________ 17

Cleaning the optics  __________________________________________________________________________ 17
Maintenance of the stand _____________________________________________________________________ 17
Replacing the fuse ___________________________________________________________________________ 17
Using the Kensington™ security slot ____________________________________________________________ 17

Digital cameras ________________________________________________________________ 18
Accessories and spare parts ______________________________________________________ 18
Warranty ______________________________________________________________________ 18
Notes _________________________________________________________________________ 19

Contents

Introduction

Thank you for purchasing a Cole-Parmer MSU-400 Series compound microscope. The MSU-400 series has been 
designed with all kinds of Educational and Life Sciences applications and great durability in mind. This resulted 
in a modern, robust and high-level microscope for advanced use, equipped with the best optical and mechanical 
components. Specific attention to production methods resulted also in an excellent price/performance ratio

Please read this manual carefully before using this product to ensure correct and safe usage

• 

The content of this manual is subject to change without notice

• 

The appearance of the actual product can differ from the models described in this manual

• 

Not all equipment mentioned in this manual has to be part of the set you have purchased

• 

All optics are anti-fungus treated and anti-reflection coated for maximum light throughput

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Summary of Contents for 78904-02

Page 1: ...Installation and Operating Instructions MSU 400 Series Compound Microscopes Models 78904 02 04 07 10 13 78904 02 78904 07 78904 13...

Page 2: ..._______________________________________________________ 11 Use of the S100x oil immersion objective_________________________________________________________ 12 Illumination____________________________...

Page 3: ...comes in direct contact with the microscope parts In that case prevention of contamination lies in the handling of the slides as long as the slides are decontaminated before use and are undamaged and...

Page 4: ...sed disinfection products for laboratories Before returning a microscope for repair or maintenance through a Cole Parmer dealer an RMA return authorization form together with a decontamination stateme...

Page 5: ...e force when turning the knobs Make sure that the microscope system can dissipate its heat fire hazard Keep the microscope away from walls and obstructions for at least approximately 15 cm Never turn...

Page 6: ...and S100x objectives are equipped with a spring mount to prevent damage to the front lens and the slide Model Type Objectives Working distance Stand Illumination Power Weight 78904 02 Binocular 4x 10...

Page 7: ...er adjustment B Trinocular tube K Slide for polarization filter C Microscope head L Nosepiece D Transport handle M Objectives E Height adjustment condenser N Stage with X Y mechanical stage F Slide pr...

Page 8: ...2 Install the objectives into the objective nosepiece from the lowest magnification to the highest in a clockwise direction from the rear side of the microscope When operating start using the low magn...

Page 9: ...illumination For optimal contrast and resolution one should follow the below procedure 1 Place a specimen on the object stage and focus using the 4x objective with a fully opened iris diaphragm 2 Tur...

Page 10: ...e field of view see image below This distance can be set by either pulling the tubes towards each other or pulling them away from each other This distance is different for each observer and thus shoul...

Page 11: ...aphragm gradually 7 The condenser is centered correctly if the image remains in the center when you open the field diaphragm and inscribed to the field of view The field K hler diaphragm see A in pict...

Page 12: ...the front lens doesn t touch the oil any longer Clean the front lens of the S100x objective 12 Always clean the front lens of the S100x objective with a piece of lens paper that is moistened with a dr...

Page 13: ...te the 10 infinity phase contrast objective into the field 10 to match the objective Take the eyepiece out of the tube and insert centering telesc telescope the dark and bright ring images should coin...

Page 14: ...reen F Fluorescence illuminator G Turret for LEDs Installation steps Take the microscope with fluorescence attachment fluorescence illuminator and head from its packaging Place the head on the fluores...

Page 15: ...escence filters Blue Green Violet and Ultraviolet mounted in the turret fig 1 D and are indicated as B G V and UV on the turret There is also a brightfield option and it is indicated as 0 on the turre...

Page 16: ...steps 1 Use objective 10x 2 Push in the field diaphragm switch the one in the front fig 2 A a light spot will be visible in the field of view 3 Adjust the centering if the light spot deviates from th...

Page 17: ...Cleaning the optics When the eyepiece lens or front lens of the 10x or S40x objective are dirty they can be cleaned by wiping a piece of lens paper over the surface circular movements When this does...

Page 18: ...roscopes come with a five 5 year factory warranty against manufacturing defects covering labor and parts Warranty does not apply for consumable accessories and parts such as bulbs batteries fuses cord...

Page 19: ..._ ___________________________________________________________________________________________________ __________________________________________________________________________________________________...

Page 20: ...2022 Cole Parmer Instrument Company LLC Toll free 1 800 323 4340 Phone 1 847 549 7600 Fax 1 847 247 2929 coleparmer com 1065Y62_M_CP_MSU 400_Series_Compound Microscopes_Manual...

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