Use of the S100x oil-immersion objective
The MSU-400 Series microscopes are equipped with an S100x N.A. 1.25 oil immersion objective. Please follow the
below instructions on how to use this objective:
1.
Remove the dust protection cap from the revolving nosepiece to mount the S100x objective
2.
Focus the image with the S40x objective
3.
Lock the slide protection handle
4.
Turn the revolving nosepiece so the S100x objective almost reaches the click-stop
5.
Put a small drop of immersion oil on the center of the slide
6.
Now turn the S100x objective so that you feel the click stop
7.
The front lens is in contact with the immersion oil
8.
Look through the eyepiece and focus the image with the fine adjustment knobs
9.
The distance between the lens of the objective and the slide is very small!
10.
In case there are small bubbles visible, turn the S100x objective a couple of times from left to right so that the
front of the objective moves in the oil and the bubbles will disappear
11.
After using the S100x objective, loosen the slide protection handle and turn the table with the course
adjustment knobs downwards until the front lens doesn’t touch the oil any longer. Clean the front lens of the
S100x objective
12.
Always clean the front lens of the S100x objective with a piece of lens paper that is moistened with a drop of
isopropanol.
13.
Clean the slide after use as well
Illumination
The illumination has the following specifications:
LED
: 3W NeoLED for binocular and trinocular models.
Power supply
: Primary 100-240 VAC, 50/60 Hz
Fuse specification
: 250V 1A
Phase contrast (model 78904-10)
Use of phase contrast
The phase contrast method was designed in 1934 by the Dutchman Frits Zernike to observe very thin or transparent
objects. This technique uses the fact that light travelling through tissue undergoes a phase shift due to diffraction. By
recombining the phase shifted light with the background light, a contrasted image appears in the eyepiece.
Using the Zernike phase contrast set
Any MSU-400 model with a Zernike phase contrast set comes with the phase contrast condenser and objectives
already mounted and centered on your microscope. If you suspect misalignment or want to check the alignment
please see the next point for “centering the phase rings”. The height of the condenser can be adjusted by turning
the rack and pinion up and down. By doing this the light beam will be focused more on the specimen for maximum
resolution.
12
