C
B
A
Centering the phase rings
The Zernike phase disc has five positions: 0 for brightfield observation (this position also has an iris diaphragm), 10, 20,
40, and 100.
These positions correspond to the respective phase contrast objectives 10x, 20x, 40x and 100x.
When the condenser is in the “0” position, the objectives can be used for brightfield observation. For phase contrast,
the condenser position should match the objective used, meaning that when the condenser is in position “40” the
objective used should also be 40x.
•
Rotate the 10× infinity phase contrast objective into the field of view, then set the Zernike phase disc to position
“10” to match the objective
•
Take the eyepiece out of the tube and insert centering telescope in its place. When looking through the
centering telescope, the dark and bright ring images should coincide with each other as shown in the pictures
below. If the ring images cannot be observed clearly, focus the centering telescope first (A) and if this does not
solve the issue then try to adjust the condenser by turning it up and down (B).
•
If the bright ring and dark ring images do not coincide as shown below, adjust the position of the ring by
moving the ring at the bottom of the condenser with the centering knobs (C). Move it until the bright and dark
ring images superimpose. Repeat for all objectives/Zernike disc positions
15
These positions correspond to the respective phase contrast objectives 10x, 20x, 40x and 100x.
When the condenser is in the “0” position, the objectives can be used for brightfield observation. For phase
contrast, the condenser position should match the objective used, meaning that when the condenser is in
position “40” the objective used should also be 40x
Rotate the 10× infinity phase contrast objective into the field of view, then set the Zernike phase disc to position
“10” to match the objective
Take the eyepiece out of the tube and insert centering telescope in its place. When looking through the centering
telescope, the dark and bright ring images should coincide with each other as shown in the pictures below. If the
ring images cannot be observed clearly, focus the centering telescope first (A) and if this does not solve the issue
then try to adjust the condenser by turning it up and down (B).
If the bright ring and dark ring images do not coincide as shown below, adjust the position of the ring by moving
the ring at the bottom of the condenser with the centering knobs (C). Move it until the bright and dark ring
images superimpose. Repeat for all objectives/Zernike disc positions
Not centered
Centered properly
Not centered
15
These positions correspond to the respective phase contrast objectives 10x, 20x, 40x and 100x.
When the condenser is in the “0” position, the objectives can be used for brightfield observation. For phase
contrast, the condenser position should match the objective used, meaning that when the condenser is in
position “40” the objective used should also be 40x
Rotate the 10× infinity phase contrast objective into the field of view, then set the Zernike phase disc to position
“10” to match the objective
Take the eyepiece out of the tube and insert centering telescope in its place. When looking through the centering
telescope, the dark and bright ring images should coincide with each other as shown in the pictures below. If the
ring images cannot be observed clearly, focus the centering telescope first (A) and if this does not solve the issue
then try to adjust the condenser by turning it up and down (B).
If the bright ring and dark ring images do not coincide as shown below, adjust the position of the ring by moving
the ring at the bottom of the condenser with the centering knobs (C). Move it until the bright and dark ring
images superimpose. Repeat for all objectives/Zernike disc positions
Not centered
Centered properly
Centered properly
13
