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Models

The Cole-Parmer MSU-400 Series compound microscopes are equipped with HWF 10x/20 mm eyepieces, and are 
available in the following brightfield and phase contrast models. Model 78904-13 includes a fluorescence attachment 
with four 5 W LEDs for fluorescence excitation from 450 to 470 nm (blue), 515 to 535 nm (green), 390 to 400 (violet) and 
360 to 370 nm (ultraviolet; and a UV protection shield.

Please note

: on coleparmer.com you can find the latest updates about MSU-400 models and accessories

Objectives

Eyepiece 

magnification

Objective 

magnification

Total m

magnification

10x

4x

40x

10x

10x

100x

10x

20x

200x

10x

40x

400x

10x

60x

600x

10x

100x

1000x

The total magnification of the microscope can be calculated by multiplying the magnification of the eyepiece with the 
magnification of the objective. The magnifications are displayed in the table. The S40x, S60x and S100x objectives are 
equipped with a spring mount, to prevent damage to the front lens and the slide

Model

Type

Objectives

Working  

distance

Stand

Illumination

Power

Weight

78904-02

Binocular

4x/10x/S40x/

S100x, semi-plan

48 to 76 mm

Rackless integrated 

X-Y mechanical stage

3 W adjustable Kohler 

NeoLED

100–240 VAC, 

50/60 Hz

14.3 lb (6.5 kg)

78904-04

Trinocular

4x/10x/S40x/

S100x, semi-plan

48 to 76 mm

Rackless integrated 

X-Y mechanical stage

3 W adjustable Kohler 

NeoLED

100–240 VAC, 

50/60 Hz

14.7 lb (6.6 kg)

78904-07

Trinocular

4x/10x/S40x/

S100x, plan

48 to 76 mm

Rackless integrated 

X-Y mechanical stage

3 W adjustable Kohler 

NeoLED

100–240 VAC, 

50/60 Hz

14.7 lb (6.6 kg)

78904-10

Trinocular

10x/20x/S40x/

S100x, plan, 

phase contrast

48 to 76 mm

Rackless integrated 

X-Y mechanical stage

3 W adjustable Kohler 

NeoLED

100–240 VAC, 

50/60 Hz

14.7 lb (6.6 kg)

78904-13

Trinocular

4x/10x/S40x/

S100x, plan

48 to 76 mm

Rackless integrated 

X-Y mechanical stage

3 W adjustable Kohler 

NeoLED; four 5 W LED 

Epi-illumination for 

fluorescence

100–240 VAC, 

50/60 Hz

14.7 lb (6.6 kg)

6

Summary of Contents for 78904-02

Page 1: ...Installation and Operating Instructions MSU 400 Series Compound Microscopes Models 78904 02 04 07 10 13 78904 02 78904 07 78904 13...

Page 2: ..._______________________________________________________ 11 Use of the S100x oil immersion objective_________________________________________________________ 12 Illumination____________________________...

Page 3: ...comes in direct contact with the microscope parts In that case prevention of contamination lies in the handling of the slides as long as the slides are decontaminated before use and are undamaged and...

Page 4: ...sed disinfection products for laboratories Before returning a microscope for repair or maintenance through a Cole Parmer dealer an RMA return authorization form together with a decontamination stateme...

Page 5: ...e force when turning the knobs Make sure that the microscope system can dissipate its heat fire hazard Keep the microscope away from walls and obstructions for at least approximately 15 cm Never turn...

Page 6: ...and S100x objectives are equipped with a spring mount to prevent damage to the front lens and the slide Model Type Objectives Working distance Stand Illumination Power Weight 78904 02 Binocular 4x 10...

Page 7: ...er adjustment B Trinocular tube K Slide for polarization filter C Microscope head L Nosepiece D Transport handle M Objectives E Height adjustment condenser N Stage with X Y mechanical stage F Slide pr...

Page 8: ...2 Install the objectives into the objective nosepiece from the lowest magnification to the highest in a clockwise direction from the rear side of the microscope When operating start using the low magn...

Page 9: ...illumination For optimal contrast and resolution one should follow the below procedure 1 Place a specimen on the object stage and focus using the 4x objective with a fully opened iris diaphragm 2 Tur...

Page 10: ...e field of view see image below This distance can be set by either pulling the tubes towards each other or pulling them away from each other This distance is different for each observer and thus shoul...

Page 11: ...aphragm gradually 7 The condenser is centered correctly if the image remains in the center when you open the field diaphragm and inscribed to the field of view The field K hler diaphragm see A in pict...

Page 12: ...the front lens doesn t touch the oil any longer Clean the front lens of the S100x objective 12 Always clean the front lens of the S100x objective with a piece of lens paper that is moistened with a dr...

Page 13: ...te the 10 infinity phase contrast objective into the field 10 to match the objective Take the eyepiece out of the tube and insert centering telesc telescope the dark and bright ring images should coin...

Page 14: ...reen F Fluorescence illuminator G Turret for LEDs Installation steps Take the microscope with fluorescence attachment fluorescence illuminator and head from its packaging Place the head on the fluores...

Page 15: ...escence filters Blue Green Violet and Ultraviolet mounted in the turret fig 1 D and are indicated as B G V and UV on the turret There is also a brightfield option and it is indicated as 0 on the turre...

Page 16: ...steps 1 Use objective 10x 2 Push in the field diaphragm switch the one in the front fig 2 A a light spot will be visible in the field of view 3 Adjust the centering if the light spot deviates from th...

Page 17: ...Cleaning the optics When the eyepiece lens or front lens of the 10x or S40x objective are dirty they can be cleaned by wiping a piece of lens paper over the surface circular movements When this does...

Page 18: ...roscopes come with a five 5 year factory warranty against manufacturing defects covering labor and parts Warranty does not apply for consumable accessories and parts such as bulbs batteries fuses cord...

Page 19: ..._ ___________________________________________________________________________________________________ __________________________________________________________________________________________________...

Page 20: ...2022 Cole Parmer Instrument Company LLC Toll free 1 800 323 4340 Phone 1 847 549 7600 Fax 1 847 247 2929 coleparmer com 1065Y62_M_CP_MSU 400_Series_Compound Microscopes_Manual...

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