The eyeshades (optional)
The eyepieces can be equipped with optional rubber eyeshades. This prevents damage to the lens, and prevents stray
light. The eyeshade can simply be slipped over the eyepiece
Connecting the power cord
The MSU-400 microscopes support a wide range of operating voltages: from 100 to 240 VAC. Please use a grounded
power connection
•
Make sure the power switch is off before connecting
•
Insert the connector of the power cord into the power socket, and make sure it connects well
•
Insert the other connector into the mains socket, and make sure it connects well. Do not bend or twist the
power cord, it will get damaged. Use the power cord that is supplied by Cole-Parmer. If it is lost or damaged,
choose one with the same specifications
Replacement of the Power Cord
If the power cord requires replacement, ensure that it is suitably rated. If in doubt consult your local dealer or qualified
electrician
Operation
Setting up the illumination
For optimal contrast and resolution one should follow the below procedure:
1.
Place a specimen on the object stage and focus using the 4x objective, with a fully opened iris diaphragm
2.
Turn light intensity to lowest position, then look through the eyepiece(s) and turn up to the comfortable light
intensity level
3.
Turn the condenser in the highest position (for phase contrast models, please set condenser to brightfield
position)
4.
Close the iris diaphragm, until it is just visible on the edge of the field of view
The microscope is properly set for use with the 4x objective. For each other magnification in brightfield use this
procedure should be repeated to ensure the best balance between contrast and resolution. Phase contrast set up is
explained later in this manual
Caution:
The maximum light intensity when using the 4x and 10x can damage the eyes!
Placing the specimen slide
1.
Push the arm of the specimen holder backwards
2.
Release the arm slowly clamping the slide with the cover glass facing up
3.
Rotating the X and Y-axis knob will move the specimen to the center for alignment with the center of the
objective
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