Operation of reflected illumination system (LED fluorescence unit)
To get the best result when observing with fluorescence please pay attention to the following:
1.
Select the fluorescence filter that matches the fluorochrome and fluorescent characteristic
2.
No autofluorescence specimen or device should be in the optical system, such as the cedar immersion liquid
which has cyan autofluorescence
3.
Always turn off the transmitted illumination before turning on the reflected illumination system
Set and adjust the fluorescence unit
The most important part of the unit is the fluorescence filters assembly. There are four fluorescence filters, Blue, Green,
Violet and Ultraviolet, mounted in the turret (fig. 1, D) and are indicated as B, G, V and UV on the turret. There is also a
brightfield option and it is indicated as 0 on the turret.
The fluorescence filters in the turret correspond with the LEDs in the fluorescence illuminator (see table). There is a
turret (fig. 1, G) on top of the fluorescence illuminator and has the same indication of B, G, V and UV as the turret for the
fluorescence filters.
Fluorescence filters
LED light source
B
B
G
G
V
V
UV
UV
When selecting the filter, turn the turret (D) to the correct filter and choose the corresponding LED by turning the
turret on the illuminator (G).
Remark:
For brightfield observation please choose position 0 on the turret (D) and switch off the
fluorescence illuminator and turn on the transmitted illumination
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