Great Bay Community College
Document
Number:
1.16.1
320
Corporate
Drive
Revision
Number:
5
Portsmouth,
NH
03801 Effective
Date:
20May09
Page 1 of 20
Title: New Brunswick BioFlo 3000 Bioreactor SOP
Approvals:
Preparer: ___Kari Britt_______________________________ Date _____15May09______
Reviewer: __Bob O’Brien_____________________________ Date _____15May09______
1.
Purpose:
Operation of the New Brunswick BioFlo 3000 Bioreactor.
2.
Scope:
Applies to growing yeast or bacteria in the New Brunswick BioFlo 3000 Bioreactor.
3.
Responsibilities:
3.1.
It is the responsibility of the course instructor /lab assistant to ensure that this SOP is
performed as directed and to update the procedure when necessary.
3.2.
It is the responsibility of the students/technicians to follow the SOP as described and to
inform the instructor about any deviations or problems that may occur while performing
the procedure.
4.
References:
4.1.
New Brunswick BioFlo 3000 Bioreactor instrumentation manual
4.2.
BioCommand Lite Software Manual
4.3.
autoclave SOP
5.
Definitions: N/A
6.
Precautions: N/A
7.
Materials:
7.1.
pH 4.0 buffer standard
7.2.
pH 7.0 buffer standard
7.3.
laboratory tissues
7.4.
silicone tubing
7.5.
PharMed tubing
7.6.
clamps
7.7.
aluminum foil
7.8.
air filters
7.9.
glass wool
7.10.
probe stand with clamps
7.11.
glycerol
7.12.
laboratory glassware detergent
7.13.
deionized water
7.14.
DO electrolyte
7.15.
3M potassium chloride (KCl) solution for pH probe storage
7.16.
bleach
7.17.
Optional: antifoaming agent (such as Antifoam A)
7.18.
personal computer to run BioCommand Lite Software and Microsoft Excel
7.19.
Bioreactor
7.19.1.
pH probe
7.19.2.
DO probe
7.19.3.
RTD temperature probe
7.19.4.
harvest tube
7.19.5.
sparger tube
7.19.6.
thermowell tube
11
