Great Bay Community College
Document
Number:
1.16.1
320
Corporate
Drive
Revision
Number:
5
Portsmouth,
NH
03801 Effective
Date:
20May09
Page 4 of 20
Title: New Brunswick BioFlo 3000 Bioreactor SOP
8.6.4.
Lower the foam probe into the foam probe port as far as it will go and then hand-
tighten the nut to the threads on the topside of the headplate.
8.7.
Connect Bioreactor to
Cabinet
8.7.1.
Place the vessel and heat exchanger base on the platform in front
of the cabinet so that the heat exchanger base fits on the three steel plugs. The
heat exchanger connectors should face the front left corner of the platform. See
Figure
1 for proper orientation of the heat exchanger.
8.7.2.
Make sure that the two-way valve of the sampling assembly is closed. The valve
is closed when the position of the two-way valve handle is perpendicular to the
sample tube, as shown in Figure 2.
8.7.3.
Connect the “Jacket Water Out” water line to the top heat exchanger connector
using the quick connects adaptors.
8.7.4.
Connect the “Jacket Water In” water line to the bottom heat exchanger connector
using the quick connects adaptors.
8.7.5.
Connect the “Condenser Water Out” water line to the top condenser connector
using the quick connects adaptors.
8.7.6.
Connect the “Condenser Water In” water line to the bottom condenser connector
using the quick connects adaptors.
8.7.7.
Turn on the water. The water valve is located under the lab bench where the
BioFlo 3000 is located. The pressure gauge should read between 15 and 20psi
when the water is turned on.
8.8.
Calibrate the pH Probe
8.8.1.
Turn on the BioFlo 3000.
8.8.2.
Select fermentation (2) and press the ENTER key.
8.8.3.
Check the pH probe for any damage. Replace the probe if damaged.
8.8.4.
Remove the shorting cap
from the top of the pH probe and connect the pH cable
to the pH probe.
8.8.5.
Press the SCREEN key to select the <Calibration> screen and then press the
ENTER key.
8.8.6.
Remove the protective cap from the bottom of the pH probe. Carefully pour the
3M potassium chloride storage solution into a test tube with a screw cap. Label
the tube as: Bioflo 3000 pH Probe Storage Solution, 3M potassium chloride. Store
in a test tube rack at room temperature until needed.
8.8.7.
Carefully immerse the pH and RTD temperature probes into a pH 7 standard
buffer solution. Allow a few minutes for the electrode to equilibrate.
8.8.8.
Use the arrow keys to select the box in the “pH” row of the “Function” column.
This box should be displaying “Read” initially.
8.8.9.
Change “Read” to “Zero” by pressing the ALTER key until “Zero” appears. Then
press the ENTER key.
8.8.10.
Use the arrow keys to select the box located in the “pH” row of the “Zero”
column. Enter “7.0” using the number key pad and then press the ENTER key.
Do this step even if “7.0” was already displayed when selecting the box.
8.8.11.
Rinse the pH and RTD temperature probes thoroughly with deionized water. Pat
them dry with a laboratory tissue.
14
