Great Bay Community College
Document
Number:
1.16.1
320
Corporate
Drive
Revision
Number:
5
Portsmouth,
NH
03801 Effective
Date:
20May09
Page 10 of 20
Title: New Brunswick BioFlo 3000 Bioreactor SOP
8.15.2.4.
Set the gases mode to DO control by selecting the box in the “Mode” row (it
is the only box available in the “Mode” row). Press the ALTER key until
“DO” is displayed and then press the ENTER key.
8.15.2.5.
Use the SCREEN key to choose the <Master> screen and then press the
ENTER key.
8.15.2.6.
To input the DO setpoint select the box in the “Set” row of the “DO2 1”
column. Use the number keypad to enter the desired setpoint, as indicated
by the process SOP and then press the ENTER key.
8.15.3.
Controlling by Agitation and % Oxygen
Note: In this mode agitation will be increased first until maximum rpm is reached and
more oxygen is required, then control by % oxygen will begin.
8.15.3.1.
Use the SCREEN key to choose the <Master> screen and then press the
ENTER key.
8.15.3.2.
Set the DO loop to P-I-D by selecting the box in the “Control” row of the
“DO2 1” column. Press the ALTER key until “P-I-D” appears and then
press the ENTER key.
8.15.3.3.
Temporarily set the agitation loop to P-I-D by selecting the box in the
“Control” row of the “Agit 1” column. Press the ALTER key until “P-I-D”
appears and then press the ENTER key.
8.15.3.4.
To set the minimum rpm select the box in the “Set” row of the “Agit 1”
column. Use the number keypad to enter the desired minimum rpm, as
indicated by the process SOP and then press the ENTER key.
8.15.3.5.
Set the agitation loop to DO control mode by selecting the box in the
“Control” row of the “Agit 1” column. Press the ALTER key until “DO”
appears and then press the ENTER key.
8.15.3.6.
To set the maximum rpm select the box in the “Set” row of the “Agit 1”
column. Use the number keypad to enter the desired maximum rpm, as
indicated by the process SOP and then press the ENTER key.
Note: The controller will automatically adjust the rpm, therefore after the
maximum value is entered the displayed set value may change immediately
afterward
8.15.3.7.
Press the SCREEN key and then use the arrow keys to choose the <Gases>
screen and then press the ENTER key.
8.15.3.8.
Set the gases mode to agitation and % oxygen control by selecting the box
in the “Mode” row (it is the only box available in the “Mode” row). Press
the ALTER key until “Ag02DO” is displayed and then press the ENTER
key.
8.15.3.9.
Use the SCREEN key to choose the <Master> screen and then press the
ENTER key.
8.15.3.10.
Navigate to the air monitor loop by selecting the column heading “Agit 1”
in the “Loop Name” row. Press the ALTER key until “Air 1” appears.
8.15.3.11.
Input the desired air pressure by selecting the box in the “Set” row of the
“Air 1” column. Use the number keypad to enter the desired air flow rate
(usually between 1.5 and 2 L/min.) and then press the ENTER key.
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