Great Bay Community College
Document
Number:
1.16.1
320
Corporate
Drive
Revision
Number:
5
Portsmouth,
NH
03801 Effective
Date:
20May09
Page 13 of 20
Title: New Brunswick BioFlo 3000 Bioreactor SOP
BT220 [semester] [year]. Record file name in the batch record, then click
“OK”.
8.17.7.16.
From the “Recipe[#].B1:Setup.[#]” window, click on the “Views” tab.
8.17.7.17.
In the “New Views:” box, double click on “Trend”.
8.17.7.18.
The “Recipe[#].B1:Trend.[#]” window should appear.
8.17.7.19.
Display trend data using the following procedure:
8.17.7.19.1.
From the “Recipe[#].B1:Trend.[#]” window, select the “Loops” tab.
8.17.7.19.2.
Click on “Setup Loop 1” (or the appropriate Loop number).
8.17.7.19.3.
From the “Select Loop” window, highlight one of the parameters to
be monitored (i.e.: Agit 1). Then click “OK”. The parameter name
chosen is automatically transferred to the “Recipe[#].B1:Trend.[#]”
window.
8.17.7.19.4.
Click “>>”.
8.17.7.19.5.
Click “Setup Loop2”.
8.17.7.19.6.
From the “Select Loop” window, highlight another parameter.
8.17.7.19.7.
Click “OK”.
8.17.7.19.8.
Click “>>”.
8.17.7.20.
Repeat step 8.17.26. until all parameters needed are added to the
“Recipe[#].B1:Trend.[#]” window. (AGIT1, DO2 1, TEMP 1 and pH 1
should always be added.)
8.17.7.21.
Set the graph range for each parameter using the following procedure.
8.17.7.21.1.
In the “Recipe[#].B1:Trend.[#]” window select the “Graphs” tab. Use
“<<” to navigate all the way back to the first parameter (Loop 1).
Enter the values listed below into the appropriate box for each
parameter then select “>>” to apply the change to the graph.
8.17.8.
Do NOT close any windows during a run.
8.18.
Inoculation Procedure
8.18.1.
Allow the bioreactor time to reach all of its setpoints.
8.18.2.
Attach the bulb to sample port (be sure there is glass wool in tube before
attaching).
8.18.3.
Verify that all of the setpoint parameters are within range and then aseptically
add the inoculum through the inoculation port per the process SOP.
8.18.4.
Take immediate sample per process SOP for baseline determination.
8.19.
Monitor Parameters per process SOP.
8.20.
Harvest Culture per process SOP.
8.21.
Shut Down Procedure
Parameter Low: High:
AGIT1 100
1100
DO21 0.0
100
TEMP1 0.0 40.0
pH1 1.0
7.0
Feed1 0 10
Feed 2
0
10
23
