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Beckman Coulter SX4750A, Instruction For Use, Installation And Assembly

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Beckman Coulter SX4750A Instruction For Use, Installation And Assembly Download Page 39

GX-TB-004EE   

1-29

SX4750A Swinging-Bucket Rotor

Care and Maintenance  

Aerosolve Cannisters

WARNING

When working with potentially hazardous materials, open cannisters in an 

appropriate hood or biological safety cabinet in case of tube breakage.

If a glass tube breaks in an Aerosolve cannister tube rack, discard and replace the O-ring and rubber 

tube cushion in the base of the rack. Imbedded glass particles that remain in the bucket or adapters 

can break tubes during subsequent runs.

SST or Corvac Tubes

If an SST or Corvac tube breaks, the tube’s silicone gel barrier material becomes laced with glass 

fragments and the tube contents contaminate the entire tube adapter and bucket (or tube rack and 

cannister).

If one of these tubes breaks in an adapter i

n a bucket

, use the following procedure.

1

Fill the bucket with water.

2

Autoclave the entire bucket and contents at 121°C for 20 minutes.

3

Decant any remaining water and dislodge the tube adapter (do not remove it) over a waste 
container.

4

Working under a hood and using plastic gloves, immerse the bucket and tube adapter (intact) 
in a beaker or similar vessel containing ethyl acetate.

*

5

Scrub the adapter with a brush and decant the ethyl acetate mixture into a waste bottle for 
proper disposal.

6

Rinse with water and air-dry the bucket and tube adapter.

If one of these tubes breaks in an 

Aerosolve cannister,

 use the following procedure.

1

Working in a biologic safety cabinet and wearing plastic gloves, open the cannister and 
immerse the cannister, lid, and contents (intact) in a beaker or similar vessel containing 6% 

sodium hypochlorite (undiluted bleach).

* Flammability hazard. Do not use in or near operating centrifuges.

Summary of Contents for SX4750A

Page 1: ...ary 2018 Instructions For Use SX4750A Swinging Bucket Rotor For Use in the Beckman Coulter Allegra X 12 Series Allegra X 14 Series and Allegra X 15R Centrifuges Beckman Coulter Inc 250 S Kraemer Blvd Brea CA 92821 U S A ...

Page 2: ...service marks products or services are trademarks or registered trademarks of their respective holders Contact Us If you have any questions contact our Customer Support Center Worldwide find us via our website at www beckmancoulter com customersupport support In the USA and Canada call us at 1 800 369 0333 Outside of the USA and Canada contact your local Beckman Coulter Representative Beckman Coul...

Page 3: ...sections have changed subsequent to revision EB Table 2 Beckman Coulter Tubes and Bottles for the SX4750A Rotor Beckman Coulter Inc SX4750A Warranty Issue ED 11 15 The following sections have changed subsequent to revision EC Symmetrical and Balanced Loading Issue EE 02 18 The following sections have changed subsequent to revision ED Description footnote on page 2 Aerosolve Cannisters footnote on ...

Page 4: ...GX TB 004EE iv Revision History ...

Page 5: ...s situation which if not avoided may result in minor or moderate injury It may also be used to alert against unsafe practices NOTE NOTE is used to call attention to notable information that should be followed during installation use or servicing of this equipment Safety Information for the SX4750A Rotor Handle body fluids with care because they can transmit disease No known test offers complete as...

Page 6: ...rriers loaded or empty to the rotor for every run Make sure that filled containers are loaded symmetrically into the rotor and that opposing containers are filled to the same level with liquid of the same density The rotor and accessories are not designed for use with materials capable of developing flammable or explosive vapors Do not centrifuge such materials in nor handle or store them near the...

Page 7: ... 5 Prerun Safety Checks 5 Installing the Rotor Yoke 6 Loading the Buckets 7 Symmetrical and Balanced Loading 7 Using Tube and Bottle Buckets 8 Using Tubes 10 Assembling Modular Disk Adapters 10 Loading Tubes in Adapters 11 Loading Adapters in the Buckets 11 Using Bottles in Adapters 11 Using Aerosolve Cannisters 12 Using Blood Bag Cups 12 Using Cell Culture Flask Adapters 14 Using Bucket Covers 14...

Page 8: ...Bottle Adapters and Blood Bag Cups 27 Cell Culture Flask Adapters 27 Decontamination 27 Sterilization and Disinfection 28 Tube Breakage 28 Aerosolve Cannisters 29 SST or Corvac Tubes 29 Storage 30 Returning a Rotor 30 Supply List 31 Replacement Rotor Parts 31 Other 31 Beckman Coulter Inc SX4750A Warranty ...

Page 9: ...ations Illustrations 1 Examples of Correctly and Incorrectly Loaded Buckets and Carriers 9 2 Assembling a Modular Disk Adapter 10 3 Blood Bag Loading Procedures 13 4 Lubricating Pivot Pin Bucket Contact Areas 26 ...

Page 10: ... Cups Available for the SX4750A Rotor 18 2 Beckman Coulter Tubes and Bottles for the SX4750A Rotor 20 3 Beckman Coulter Labware for Use with Multiwell Plate Carriers 23 4 Commercially Available Labware for Use with Multiwell Plate Carriers 24 ...

Page 11: ...ier and cover 360 grams Total maximum allowable imbalance of opposing loads 50 grams Maximum rotor capacity 3 liters Approximate acceleration time 80 seconds Approximate deceleration time 81 seconds Weight of fully loaded rotor buckets with covers 15 4 kg 33 9 lb Rotor yoke material stainless steel Bucket and carrier material anodized aluminum a The critical speed range is the range of speeds over...

Page 12: ... swing out to horizontal position during centrifugation Transparent covers made of high impact plastic have been tested to demonstrate containment of microbiological aerosols under normal conditions of the associated Beckman Coulter centrifuge when used and maintained as instructed A captive tie down device is used to secure the rotor to the drive shaft during centrifugation The ARIES Automated Ro...

Page 13: ...blue adapter allowing all the tubes to be decanted at once Additionally 1 5 mL Microfuge tubes can be run using a special plate that fits on top of the blue adapter Both of these accessories are described in Table 1 Beckman Coulter tubes and bottles available for use in the rotor buckets are described in Table 2 Bottle Adapters Bottles are supported in polypropylene adapters that fit inside the ro...

Page 14: ...s Cell Culture Flask Adapters Specially designed EPDM ethylene propylene adapters can be used to centrifuge Corning 25 cm2 two per bucket or 75 cm2 one per bucket canted neck cell culture flasks Using these adapters the separation and concentration step of the cell culturing process can be accomplished in the flask Eliminating the need to transfer cultures into tubes for centrifugation and back in...

Page 15: ...rifuge operation NOTE Although rotor components and accessories made by other manufacturers may fit in the SX4750A rotor their safety in this rotor cannot be ascertained by Beckman Coulter Use of other manufacturers components or accessories in the SX4750A rotor may void the rotor warranty and should be prohibited by your laboratory safety officer Only the components and accessories listed in this...

Page 16: ...306812 CAUTION Never drop the rotor yoke onto the centrifuge drive shaft The drive shaft can be damaged if the rotor is forced sideways or dropped onto it 2 Carefully lower the yoke straight down onto the centrifuge drive shaft Be sure the yoke is properly seated on the shaft 3 Turn the tie down T bar to the right clockwise to tighten the yoke firmly on the drive shaft NOTE If the rotor yoke is le...

Page 17: ...rrier Modular disk adapters are also sold in weight matched sets However there are variances in weight between sets as well as variance in weight between previously purchased adapters To prevent accidental imbalance it is important to keep matched sets of buckets as well as matched sets of adapters together and to weigh other adapters to be sure they are approximately the same Markingmatched adapt...

Page 18: ...respect to the pivotal axis of the bucket and opposing carriers should contain similar loads NOTE The ARIES rotors are designed to accommodate imbalances of up to 50 grams It is not necessary to carefully balance opposing tubes as in regular rotors In fact doing so may result in vibration during early acceleration as the ARIES device adjusts to equilibrium Using Tube and Bottle Buckets Whether you...

Page 19: ...kets and Carriers CORRECT INCORRECT Balanced Load Unbalanced Load 1 5 4 3 2 6 7 3 8 1 Balanced Load 2 Unbalanced Load 3 Pivotal Axis of Bucket 4 Center of Rotation 5 CORRECT 6 Examples of Symmetrically Loaded Multiwell Plates load opposite plates the same way 7 INCORRECT 8 Examples of Nonsymetrically Loaded Multiwell Plates ...

Page 20: ... before or after they are installed on the rotor yoke In either case we recommend filling the appropriate labware first and then loading the labware into the buckets Using Tubes Assembling Modular Disk Adapters Refer to Table 1 to determine which color adapter is required for the tubes you are using and the number of disks needed to support the length of the tubes 1 Select the appropriate adapter ...

Page 21: ... position a bucket cover over the tubes checking that tubes do not touch the cover If all positions in an adapter are not filled load the adapter symmetrically with respect to its pivotal axis see Symmetrical and Balanced Loading above Loading Adapters in the Buckets After placing tubes in the disk adapters lower the adapters into the buckets so that the bails line up with the rotor pivot pins If ...

Page 22: ...cluded in the Aerosolve cannister kits 359232 Bucket covers cannot be used with Aerosolve cannisters Using Blood Bag Cups NOTE This rotor is suitable for obtaining cell free plasma or for cell packing The yellow 90 mm cup 356856 accommodates up to two single bags or one double pack the orange 97 mm cup 356857 supports either one triple or one quad pack Do not pour liquid directly into blood bag cu...

Page 23: ...n weight balance if necessary 4 Place loaded cups into rotor buckets To reduce the possibility ofbagbreakage align theblood bagseam with the rotor pivot pins see Figure 3 with the label facing out 5 Place bucket covers on rotor buckets to prevent blood bags or tubing from contacting the rotor yoke during centrifugation 1 Blood Bag Label 2 Blood Bag Seam Aligned with Pivotal Axis of Bucket 3 Ports ...

Page 24: ...tion GX TB 007 for 25 cm2 flask adapter information or GX TB 006 for 75 cm2 flask adapters These publications are supplied with the respective adapters NOTE A dry release agent is used to facilitate removal of cell culture flask adapters from buckets following centrifugation To avoid compromising the bucket cover bioseal the release agent should not be allowed on the bucket O ring When running cel...

Page 25: ...ell Plate Carriers If only two filled multiwell plate carriers are run install them opposite each other in the rotor and run two additional carriers or buckets they can be empty to prevent rotor imbalance 1 Insert the filled plate s into the carriage If using two or three Beckman Coulter plates per carrier place a 96 well cap strip see Table 3 between the plates to prevent breakage during centrifu...

Page 26: ...wo positions filled If two tube and bottle buckets and two multiwell plate carriers are run position the similar buckets opposite each other Select multiwell plate carrier speed when running mixed sets 6 Gently swing the carriers to ensure that they are properly seated on the pivot pins Operation 1 Refer to the instrument instruction manual for centrifuge operation NOTE When using multiwell plate ...

Page 27: ...r uses labware listed in Table 1 Table 2 and Table 3 RCF limitations for some non Beckman Coulter multiwell plates are shown in Table 4 Temperature Limits Beckman Coulter plastic tubes and bottles have been centrifuge tested for use at temperatures between 2 and 25 C For centrifugation at other temperatures pretest tubes under anticipated run conditions If plastic containers are frozen before use ...

Page 28: ...n 15 20 18 14 56 184 7 4659 7 359473 359152 lt green conical 30 50 30 4 16 191 4817 5 359475 359154 yellow 50 29 7 28 184 7 4659 6 359474 359153 dk blue 50 35 4 16 184 7 4659 7 359476 359155 brown 100 44 2 8 184 7 4659 3 359477 359156 tube decanter 3 5 10 12 37 148 1 343108a each 1 5 mL adapter plate 1 5 1 8 11 26 104 1 354511a each a Tube retainers and adapter plates are sold individually SOLID A...

Page 29: ...lene Color Code Nom Tube Vol mL Nom Tube Dia mm Max No Tubes per Adapter Max No Tubes in Rotor rmax at Adapter Base mm RCF at Maximum Speed g a Adapter Part Number Set of Two Set of Four white 1 5 11 24 96 174 4389 354495 each blue 3 5 12 24 96 174 4389 359482 359160 tan 5 12 24 96 180 4530 359489 358993 orange 10 14 18 72 175 4414 359483 359161 purple 12 3 5 16 12 12 6 48 24 177 178 4469 4490 359...

Page 30: ...Set of Two Set of Four BOTTLE ADAPTERS polypropylene Color Code Nominal Bottle Volume mL Nominal Bottle Diameter mm Maximum Number Bottles in Rotor rmax at Bottle Bottom mm RCF at Maximum Speed g Adapter Part Number qty one orange conical 230 62 4 195 1 4921 356983 use with 349946 yellow 250 62 4 195 1 4921 349946 warm red conical 250a 62 4 203 2 5125 349849 light purpleb 500 70 4 200 2 6050 34994...

Page 31: ...m 1 5 green polypropylene 356092 359469 354511c 354495b 359148 11 38 mm 1 5 yellow polypropylene 356093 359469 354511c 354495b 359148 11 38 mm 1 5 orange polypropylene 356094 359469 354511c 354495b 359148 11 38 mm 1 5 polypropylene cap separate 343169 359469 354511 354495b 359148 11 39 mm 1 8 white polyethylene 340196 359469 354511 354495b 359148 29 103 mm 50 polypropylene 357005 359474 359486b 35...

Page 32: ...f Two Set of Four 29 104 mm 50 polycarbonate with cap assembly 361693 359474 359486 359153 359164b 29 104 mm 50 polycarbonate with screw cap 357002 359474 359486b 359153 359164b 29 104 mm 50 polypropylene with cap assembly 357001 or 361694 359474 359486b 359153 359164b 29 104 mm 50 polypropylene with screw cap 357003 359474 359486b 359153 359164b 28 5 107 mm 50 Teflon with high speed screw cap 363...

Page 33: ...se with Multiwell Plate Carriers Description Volume Part Number Available Accessory Description Part Number Multiwell plate 96 well nonsterile 300 L well 609844a pkg 100 Cap strip nonsterilea 267002 pkg 10 Cap strip sterilea 267005 pkg 10 Aluminum foil lidb 538619 pkg 100 Deep well polystyrene plate 96 well nonsterile 1 mL well 267001 pkg 24 Cap strip nonsterilea 267002 pkg 10 Cap strip sterilea 2...

Page 34: ... multiwell plate carriers in an Allegra X 15R 4300 RPM for tube and bottle buckets or 4000 RPM for the multiwell plate carriers in an Allegra X 14 series or 3750 RPM for either buckets or carriers in an Allegra X 12 series centrifuge a Caps are optional however if stacking two or three multiwell plates use cap strips between plates for speeds greater than 2700 RPM b Requires soft rubber roller 4 i...

Page 35: ...equently aluminum oxide or heavy discoloration If any of these signs are evident do not run the rotor Contact your Beckman Coulter representative for information about the Field Rotor Inspection Program and the rotor repair center 2 Approximately every 400 runs and after cleaning and or autoclaving wipe the rotor pins and pin sockets see Figure 4 with a paper towel then coat the sockets with Paint...

Page 36: ...vers and multiwell plate carriers and covers in a mild detergent such as Beckman Solution 555 339555 that won t damage the rotor The Rotor Cleaning Kit 339558 contains two plastic coated brushes and two quarts of Solution 555 for use with rotors and accessories Dilute the detergent 10 to 1 with water NOTE Do not wash rotor components in a dishwasher Do not soak in detergent solution for long perio...

Page 37: ...pters and Blood Bag Cups 1 Use a mild detergent such as Beckman Solution 555 339555 diluted 10 to 1 with water and a soft brush to clean bottle adapters and blood bag cups 2 Thoroughly rinse with water and air dry upside down Cell Culture Flask Adapters Refer to publication GX TB 007 25 cm2 flask adapters or GX TB 006 75 cm2 flask adapters for cell culture flask adapter cleaning instructions Decon...

Page 38: ... new air vent filter 368148 into each cover Ethanol 70 or hydrogen peroxide 6 may be used on all rotor components including those made of plastic Bleach sodium hypochlorite may be used but may cause discoloration of anodized surfaces Use the minimum immersion time for each solution per laboratory standards While Beckman Coulter has tested these methods and found that they do not damage the rotor o...

Page 39: ...eaks in an adapter in a bucket use the following procedure 1 Fill the bucket with water 2 Autoclave the entire bucket and contents at 121 C for 20 minutes 3 Decant any remaining water and dislodge the tube adapter do not remove it over a waste container 4 Working under a hood and using plastic gloves immerse the bucket and tube adapter intact in a beaker or similar vessel containing ethyl acetate ...

Page 40: ...urn original purchase order number billing number and shipping number if possible name and email address of the person to be notified upon receipt of the rotor or accessory at the factory name and email address of the person to be notified about repair costs etc To protect our personnel it is the customer s responsibility to ensure that all parts are free from pathogens and or radioactivity Steril...

Page 41: ... pkg 8 961648 Tube and bottle bucket cover latch assembly 360587 Multiwell plate carrier set of 2 392806 Multiwell plate carrier cover set of 2 393071 Multiwell plate carriage set of 2 392873 Multiwell plate support pad set of 4 392872 Bucket carrier cover air vent filter set of 60 368148 Tie down T bar 369718 Adapters see Table 1 Tubes and bottles see Table 2 Multiwell plate labware see Table 3 S...

Page 42: ... 4 large 4 small 344117 344118 344119 344120 344121 Blood bag cup 90 mm yellow polypropylene 356856 Blood bag cup 97 mm orange polypropylene 356857 Balancing pads six pads 3 grams each red 358365 Cell culture flask adapter 75 cm2 pkg of 2 369292 Cell culture flask adapter 25 cm2 pkg of 2 369295 Rotor Cleaning Kit 339558 Solution 555 1 qt 339555 Silicone vacuum grease 1 oz 335148 Spinkote lubricant...

Page 43: ...ers the rotor only and Beckman Coulter shall not be liable for damage to accessories or ancillary supplies including but not limited to i tubes ii tube caps iii tube adapters or iv tube contents This warranty is void if the rotor has been subjected to customer misuse such as operation or maintenance contrary to the instructions in the Beckman Coulter rotor or centrifuge manual This warranty is voi...

Page 44: ...GX TB 004EE Warranty 2 Beckman Coulter Inc SX4750A Warranty ...

Page 45: ......

Page 46: ...n Installation Operation Troubleshooting Care and Maintenance Available in hard copy or electronic pdf by request Additional References Chemical Resistances for Beckman Coulter Centrifugation Products IN 175 Use and Care of Tubes and Bottles IN 192 Using Adapters for Corning 25 cm2 Cell Culture Flask in SX4750 and SX4750A Rotors GX TB 007 Using Adapters for Corning 75 cm2 Cell Culture Flask in SX4...

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