GX-TB-004EE
1-7
SX4750A Swinging-Bucket Rotor
Loading the Buckets
Loading the Buckets
For runs at other than room temperature, refrigerate or warm the rotor and precool the centrifuge
beforehand for fast equilibration.
WARNING
Handle body fluids with care because they can transmit disease. No known test
offers complete assurance that such fluids are free of micro-organisms. Some of
the most virulent—Hepatitis (B and C) viruses, HIV (I–V), atypical mycobacteria,
and certain systemic fungi— further emphasize the need for aerosol protection.
Handle other infectious samples according to good laboratory procedures and
methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment.
Do not run toxic, pathogenic, or other hazardous materials in this rotor without
taking all appropriate safety precautions. Biosafe containment should be used
when Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual
) are handled; materials of a higher group require
more than one level of protection.
Symmetrical and Balanced Loading
Beckman Coulter supplies buckets and carriers in weight-matched sets to make balancing easier
(the weight and retirement date are marked on the side of each bucket or carrier). Modular disk
adapters are also sold in weight-matched sets. However, there are variances in weight between sets,
as well as variance in weight between previously purchased adapters. To prevent accidental
imbalance it is important to keep matched sets of buckets, as well as matched sets of adapters,
together and to weigh other adapters to be sure they are approximately the same. Marking matched
adapter sets will help you keep them together.
NOTE
The light purple 500-mL adapters (349945) replace the previous tan adapters. There is a weight
difference between the old tan adapters and the current purple adapters. If you run a mix of the purple
and tan adapters, be sure to run matching colors in opposing positions or rotor imbalance could occur.
To ensure optimal performance and stability, the rotor must be loaded symmetrically. Two factors
affect symmetric loading:
1.
The buckets or carriers must be loaded symmetrically with respect to their pivotal axes
(see
Figure 1
).
2.
The rotor should be loaded symmetrically with respect to its center of rotation. However,
unlike other rotors, it is not necessary to balance opposing loads carefully.
For best results, load opposing buckets or carriers with the same type of labware. Unlike other
rotors, the ARIES rotors do not require careful balancing of opposing loads. Imbalances of up to 50
grams can be accommodated. Do not exceed the rated maximum load for buckets (1000 grams each
for tube-and-bottle buckets; 360 grams each for multiwell-plate carriers).
Summary of Contents for SX4750A
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