Beckman Coulter SX4750A, Instruction For Use, Installation And Assembly

Page: 34 / 46

GX-TB-004EE
1-24
SX4750A Swinging-Bucket Rotor
Operation
Run Speeds
The centrifugal force at a given radius in a rotor is a function of speed. Comparisons of forces
between different rotors are made by comparing the rotors’ relative centrifugal fields (RCF). When
rotational speed is adjusted so that identical samples are subjected to the same RCF in two different
rotors, the samples are subjected to the same force. The RCF at each speed is automatically
calculated by the centrifuge software; if the RCF is entered, the centrifuge calculates the equivalent
RPM (revolutions per minute). Run speeds must be reduced in the following circumstances:
• If the weight of the load in a tube-and-bottle bucket exceeds 1000 grams, or if the solution
density is more than 1.2 g/mL, reduce the maximum allowable run speed according to the
following equation:
•
If the weight of the load in a
multiwell-plate carrier
exceeds 360 grams, reduce the maximum
allowable run speed according to the following equation:
Do not select rotational speed in excess of:
•
4750 RPM for tube-and-bottle buckets or 4450 RPM for multiwell-plate carriers in an
Allegra X-15R,
•
4300 RPM for tube-and-bottle buckets or 4000 RPM for the multiwell-plate carriers in an
Allegra X-14 series, or
•
3750 RPM for either buckets or carriers in an Allegra X-12 series centrifuge.
a. Caps are optional; however, if stacking two or three multiwell plates, use cap strips between plates for speeds greater than 2700 RPM.
b. Requires soft rubber roller (4-in.), part number 538618, for installation.
Table 4 Commercially Available Labware for Use with Multiwell-Plate Carriers
Description Volume Part Number Required Accessory
Description Part Number
BD Falcon
a
96-well flat-bottom assay
plate, clear polystyrene, standard
surface, nonsterile
300

L/well
BD Falcon 353915
b
(5/bag, 50/case)
— —
BD Falcon 96-well flat-bottom ELISA
plate, clear polystyrene, enhanced
surface, non sterile
300

L/well
BD Falcon 353279
b
(25/sleeve, 100/case)
— —
BD Falcon 96-well flat-bottom assay
plate, clear polystyrene, standard
surface, nonsterile
300

L/well
BD Falcon 353228
b
(10/bag, 60/case)
— —
a. BD Falcon is a trademark of Becton, Dickinson and Company.
b. These plates can be run at 4450 RPM (4063

g ), up to four plates per carrier, without lids or cap strips.
reduced maximum speed = (4750 RPM)
1000 grams
heaviest load in grams
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reduced maximum speed = (4450 RPM)
360 grams
heaviest load in grams
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