Beckman Coulter SX4750A, Instruction For Use, Installation And Assembly

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GX-TB-004EE
1-8
SX4750A Swinging-Bucket Rotor
Loading the Buckets
It is not necessary to completely fill all tubes
*
or positions in buckets; however, partially filled
buckets must be balanced with respect to the bucket pivotal axis (see Figure 1 ). In multitube
adapters, each tube should be placed so that its weight is balanced by a tube in a diametrically
opposite position across the pivotal axis in the same adapter. Adapters placed in opposing buckets
should also be filled the same way.
In multiwell plate carriers, samples should be loaded into the wells symmetrically with respect to
the pivotal axis of the bucket, and opposing carriers should contain similar loads.
NOTE
The ARIES rotors are designed to accommodate imbalances of up to 50 grams. It is not necessary to
carefully balance opposing tubes as in regular rotors. In fact, doing so may result in vibration during early
acceleration as the ARIES device adjusts to equilibrium.
Using Tube and Bottle Buckets
Whether you are running tubes, bottles, cell culture flasks, or blood bags, you must load the buckets
symmetrically around the center of rotation and each bucket must be loaded symmetrically with
respect to its pivotal axis (see Symmetrical and Balanced Loading , above).
* Fill tubes at least half full. Speed reduction may be required for fill volumes below 75 percent.
1. At Rest 2. At Speed
1 2