BD FACSymphony A3, User Manual

Page: 96 / 128

User’s Guide 96
• In the FITC vs PE plot, draw a gate around the
FITC-positive population. Name the population FITC
positive in the population hierarchy.
• In the FITC vs PE plot, draw a gate around the PE-positive
population. Name the population PE positive in the
population hierarchy.
• In the FITC vs PerCP-Cy5.5 plot, draw a gate around the
PerCP-Cy5.5-positive population. Name the population
PerCP-Cy5.5 positive in the population hierarchy.
• In the FITC vs APC plot, draw a gate around the APC-
positive population. Name the population APC positive in
the population hierarchy.
11. Format the statistics view.
a. Right-click the statistics view and select Edit Statistics
View.
b. Click the Header tab and select the Specimen Name and
Tube Name checkboxes.
c. Click the Populations tab and select all populations except
All Events. Clear the %Parent , %Total , and #Events
checkboxes.
d. Click the Statistics tab and select the mean for each of the
fluorescence parameters.