BD FACSymphony A3, User Manual

Page: 89 / 128

Chapter 6: Recording and analyzing data 89
Preparing the workspace
Introduction This topic describes how to prepare the workspace and apply
application settings to your experiment before recording data.
Procedure To prepare the workspace:
1. Using the Browser toolbar, create a new specimen in
MyExperiment and rename it FourColorBeads .
2. Create two tubes for the FourColorBeads specimen. Rename
the tubes Beads_001 and Beads_002 .
3. Expand the Global Worksheets folder in MyExperiment to
access the default global worksheet, and rename the worksheet
MyData .
4. On the MyData worksheet, create the following plots for
previewing the data:
• FSC vs SSC
• FITC vs PE
• FITC vs PerCP-Cy5.5
• FITC vs APC
Applying saved
application settings
to a new
experiment When applications settings are applied to an experiment, the
cytometer settings are updated with the parameters included in the
application settings, optimized PMT voltages, threshold settings,
area scaling factors, and window extension values.
To apply saved application settings to your experiment:
1. Right-click the experiment-level Cytometer Settings and select
Application Settings > Apply.