BD FACSymphony A3, User Manual

Page: 105 / 128

Chapter 7: Troubleshooting 105
No events in
acquisition display
and RUN button is
orange
No fluorescence
signal
Possible causes Recommended solutions
RUN is not activated Press the RUN button.
Sample tube is not installed
or is not properly seated Install the sample tube correctly on the
SIP.
Sample tube is cracked Replace the sample tube.
Waste tubing line is
disconnected from the waste
container tank Connect the waste tubing line to the
waste container tank.
Sheath container is not
pressurized

Ensure that the sheath container lid
and all connectors are securely
seated.

Inspect the sheath container O-ring
inside the lid and replace it if
necessary. See
Cleaning or
replacing the sheath gasket
(page 62)
Bal seal is worn Replace the Bal seal. See Changing the
Bal seal (page 58) .
Air leak at sheath container Ensure that the sheath container lid
and all connectors are securely seated.
Sheath container is empty Fill the sheath container.
Air in sheath filter Purge the filter. See Removing air
bubbles (page 37) .
Possible causes Recommended solutions
Incorrect fluorochrome
assignment Make sure that the cytometer
configuration in the software matches
the optical filters in the cytometer and
the configuration is as expected.
Laser is not functioning Call your BD service representative.