BD FACSCanto II, Reference Manual, Page: 99 / 158

Chapter 5: Troubleshooting 99
Tube does not load, or
sample tube does not fit
snugly on SIT Improper tubes used Use only uncapped 12 x 75-mm
BD Falcon polystyrene test tubes,
BD Trucount tubes, and BD FACS
7-color setup beads tubes. See
System Requirements on page 31.
Defective or cracked tube • Transfer sample to new tube.
• Make sure you are using
appropriate tubes. See System
Requirements on page 31.
Bal seal improperly installed
or worn Reinstall or replace the Bal seal.
See Replacing the Bal Seal on
page 79.
Liquid leakage around
cytometer base Interior valve failure
1
Turn off the cytometer power.
2
Clean up the liquid, using
proper precautions.
3
Call BD Biosciences.
QC fails after Long
Clean Residual BD FACSClean
solution in lines Run Fluidics Startup to flush the
system with sheath fluid. Repeat
until QC passes.
Low laser power
indication Flow cell access door open Close the door completely.
Laser power output is below
requirement Call BD Biosciences.
Instrument Troubleshooting (continued)
Observation Possible Causes Recommended Solutions