BD FACSCanto II, Reference Manual, Page: 116 / 158

116 BD FACSCanto II Flow Cytometer Reference Manual
Figure A-10 Excitation and emission spectra of PE-Cy7
Similarly, the 655 LP mirror for PMT B will reflect light with a wavelength of less
than 655 nm to the next PMT. Light with a longer wavelength will pass through
to another LP filter (670 nm) that further blocks shorter wavelength light. Light
from dyes such as PerCP-Cy5.5 pass through this filter.
The beam continues around the array, with different light spectrum ranges
collected at successive PMTs. Side scatter signals (blue laser light that was
deflected by the internal irregularities of a particle) are collected at PMT F.
blue laser
light
bandpass
filter
excitation
emission