SEC2000 Operating Manual
SEC2000 Spectra System
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52
6-2. Quantitative Analysis with Calibration Method
6-2-1. Calibration method by calculating the absorbance area
The measuring results are quantitatively analyzed by using the absorbance area of the
selected wavelength range after calibrating the baseline. It is possible only in
absorbance mode.
①
Set the spectrometer system as shown in Fig. 6-2-1.
<Fig. 6-2-1> Configuration for an absorbance experiment.
②
A reference spectrum and a dark spectrum are measured in the same procedure as
absorbance measurement. Put a standard sample into the cuvette holder in
absorbance mode, then the screen will become as Fig. 6-2-2. The following
procedures make 1 mM potassium ferricyanide as an example.
<Fig. 6-2-2> Absorbance of 1 mM K
3
Fe(CN)
6
solution.
