BAS SEC2000 Spectra System, Operating Manual

Page: 56 / 66

SEC2000 Operating Manual
SEC2000 Spectra System
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56
②
A reference spectrum and a dark spectrum are measured in the same procedure
as absorbance measurement. Put a standard sample into the cuvette holder in
absorbance mode, the screen will become as Fig. 6-2-9. The following
procedures make 1 mM potassium ferricyanide as an example.
<Fig. 6-2-9> Absorbance of 1 mM K
3
Fe(CN)
6
solution.
③
Open the “ Quantitative Analysis ” window by “ View ”/“ Spectrum Tool Bar ”/
check the “ Quantitative Analysis ”. The window of “Quantitative Analysis” is
shown on the right side of the main screen (Fig. 6-2-9).
④
Set parameters (Fig. 6-2-10)

Set the file name and folder of saving calibration curve by “ Save ”.

Set the peak wavelength of the sample by “ Wavelength_ nm ”, we set 413 nm
for the ferricyanide.

Set the unit of solution concentration by “ Con. ”, we choose “ mM ”.

Set the unit of absorbance by “ Intensity ”, we choose “ O.D. ”.