SEC2000 Operating Manual
SEC2000 Spectra System
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35
Wait for 20 to 30 minutes until the lamps are stabilized.
③
Open the software and click “
Go
” icon, the spectrum signal will appeared. On
Scope Mode, put the standard reflective material into the cuvette holder, and open
the light source shutter fully.
④
Adjust the Integration Time to control the maximum value of the Y axis of the graph
(less than 16,000).
⑤
By clicking the “
Reference
” icon in the program, the spectrum is obtained at the
maximum light intensity. Save the reference spectrum by “
File
”/”
Save
”/
“
Reference
”.
⑥
Close the light source shutter (Do not close the power switch), and click the “
Dark
”
icon in the program to obtain a spectrum under the minimum conditions without
light. Save the dark spectrum by “
File
”/”
Save
”/”
Dark
”.
⑦
Put the sample into the cuvette holder, and open the light source shutter fully. Click
the “
Reflectance Mode
” icon to change the Mode. Save the spectrum by
“
File
”/”
Save
”/”
Processed
” or “
Sample
”.
5-5-2. Reflectance Measurement 2
In this measurement, the Reflection probe is used.
The reflectance is measured with the following procedure.
<Fig. 5-5-2> Configuration example of reflectance measurement using reflection probe.
Reflectance Standard
SEC2000-UV/VIS
SEC2000-D
H
Sample
Computer
Probe holder
Reflection Probe
