SEC2000 Operating Manual
SEC2000 Spectra System
www.als-japan.com
55
⑧
Click “
Next
”, on the step of ”
Quantitative Analysis-Analysis
”, to measure the
absorbance with unknown concentration of sample, put a sample into the cuvette
holder, move your mouse to “
Absorb.
” column, and click “
Get Absorb.
” Icon. Then
the concentration of the unknown sample will be calculated and shown in
“
Concent.
” column.
<Fig. 6-2-7> Quantitative analysis with calibration method.
The unknown concentration of the sample is shown in open circle.
6-2-2. Calibration method by calculating the peak absorbance.
Analyze the concentration of a sample by making calibration line with the peak
absorbance of standard solutions. It is possible only in absorbance mode.
①
Set the spectrometer system as shown in Fig. 6-2-8.
<Fig. 6-2-8> Configuration for an absorbance experiment
