BAS SEC2000 Spectra System, Operating Manual

Page: 33 / 66

SEC2000 Operating Manual
SEC2000 Spectra System
www.als-japan.com
33
<Fig 5-4-1>
①
First, the light source and detector of SEC2000 are set as shown in Fig. 5-4-1.
②
The power supply is switched on and turned ON the UV lamp and halogen lamp.
Wait for 20 to 30 minutes until the lamps are stabilized.
③
Open the software and click “ Go ” icon, the spectrum signal will appear. On Scope
Mode, put the reference sample into the cuvette holder, and open the light source
shutter fully.
④
Adjust the Integration Time to control the maximum value of the Y axis of the graph
(less than 16,000).
⑤
By clicking the “ Reference ” icon in the program, the spectrum is obtained at the
maximum light intensity. Save the reference spectrum by “ File ”/” Save ”/
“ Reference ”.
⑥
Close the light source shutter (Do not close the power switch), and click the “ Dark ”
icon in the program to obtain a spectrum under the minimum conditions without
light. Save the dark spectrum by “ File ”/” Save ”/” Dark ”.
⑦
Put the sample into the cuvette holder, and open the light source shutter fully. Click
the “ Absorbance Mode ”/” Transmittance Mode ” icon to change the Mode. Store
the spectrum by “ File ”/” Save ”/” Processed ” or “ Sample ” to save an absorbance or
transmittance spectrum.
Notes
When absorbance/transmittance is saved by “ File ”/” Save ”/” Processed ”, the data of
absorbance/transmittance is displayed only absorbance/transmittance mode. However,
if absorbance/transmittance is saved “ File ”/” Save ”/“ Sample ”, the saved data is
convertible for other mode.