EF600 Instruction Manual
Asymptote Ltd.
St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK
Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: [email protected]
2
Contents
1.
INTRODUCTION ................................................................................................ 5
1.1
W
ARNING
.............................................................................................................. 6
1.2
S
AFETY PRECAUTIONS
........................................................................................... 6
1.3 M
AINTENANCE
....................................................................................................... 6
2.
INSTALLING THE FREEZER UNIT ............................................................... 7
2.1 U
NPACKING
........................................................................................................... 7
2.2
P
OSITIONING THE FREEZER
.................................................................................... 7
2.3
E
LECTRICAL CONNECTION
.................................................................................... 7
2.4 S
WITCHING THE FREEZER ON
................................................................................. 7
4.
USER INTERFACE (EF600-01, EF600-10) ..................................................... 10
4.1 S
ELECTING THE LOGGING INTERVAL
(EF600-01,
EF600-10)
AND FREEZING
PROFILE
(EF600-10) ............................................................................................ 10
4.2 L
OG FILE NAMES
(EF600-01,
EF600-10) ............................................................. 11
5.
OPERATING PROCEDURE FOR THE EF600 FREEZER ......................... 13
5.1 P
RE
-
OPERATION CHECKS
...................................................................................... 13
5.2 P
REPARING THE NUCLEATING TOOL
..................................................................... 13
5.3 P
REPARING THE STRAWS
...................................................................................... 13
5.4
L
OADING THE STRAWS
........................................................................................ 13
5.5 S
TARTING THE DATA LOGGING
(EF600-01,
EF600-10) ....................................... 14
5.6 S
TARTING THE FREEZING CYCLE
.......................................................................... 14
5.7
S
AMPLE NUCLEATION
.......................................................................................... 15
5.8 E
ND OF CYCLE
..................................................................................................... 15
5.9 S
TOPPING THE DATA LOGGING
............................................................................. 16
5.10 R
ESTARTING THE CYCLE
...................................................................................... 16
5.11 C
LEANING THE UNIT
............................................................................................ 16
6.
FREEZING CYCLES ........................................................................................ 17
6.1 H
UMAN EMBRYO CRYOPRESERVATION
................................................................ 17
6.2 H
UMAN OOCYTE CRYOPRESERVATION
................................................................. 17
6.3 H
UMAN BLASTOCYST CRYOPRESERVATION
......................................................... 18
6.4 H
UMAN EMBRYONIC STEM CELL CRYOPRESERVATION
......................................... 18
6.5 H
UMAN SPERMATOZOA CRYOPRESERVATION
–
LINEAR COOLING
........................ 19
6.6 H
UMAN SPERMATOZOA CRYOPRESERVATION
–
NON
-
LINEAR COOLING
................ 20
7.
REFERENCES .................................................................................................... 21
