EF600 Instruction Manual
Asymptote Ltd.
St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK
Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: [email protected]
18
•
Start temperature
20°C
•
Cool
from 20°C to -8°C at 2°C min
-1
•
Hold for nucleation at -8°C for 10 minutes
•
Cool
from -8°C to -30°C at 0.3°C min
-1
•
Cool
from -30°C to -100°C at 10°C min
-1
•
Hold
at -100°C
-100
-80
-60
-40
-20
0
20
00:00
00:20
00:40
01:00
01:20
01:40
02:00
Time (hh:mm)
Te
m
pe
ra
tur
e
(
°C
)
Figure 6.2 – Pre-programmed human oocyte freezing cycle
6.3
Human blastocyst cryopreservation
•
Start temperature
20°C
•
Cool
from 20°C to -7°C at 1°C min
-1
•
Hold for nucleation at -7°C for 10 minutes
•
Cool
from -7°C to -30°C at 0.3°C min
-1
•
Hold
at -30°C
-30
-20
-10
0
10
20
00:00
00:20
00:40
01:00
01:20
01:40
02:00
Time (hh:mm)
Te
m
pe
ra
tur
e
(
°C
)
Figure 6.3 – Pre-programmed human blastocyst freezing cycle
6.4
Human embryonic stem cell cryopreservation
