EF600 Instruction Manual
Asymptote Ltd.
St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK
Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: [email protected]
20
6.6
Human spermatozoa cryopreservation – non-linear cooling
•
Start temperature
20°C
•
Cool
from 20°C to -7°C at 2°C min
-1
•
Hold for nucleation at -7°C for 10 minutes
•
Cool
non-linear from -7°C to -100°C at an
approximate rate of 10°C min
-1
•
Hold
at -100°C
-100
-80
-60
-40
-20
0
20
00:00
00:10
00:20
00:30
00:40
Time (hh:mm:ss)
Te
m
pe
ra
tur
e
(
°C
)
Figure 6.6 – Pre-programmed human spermatozoa non-linear freezing cycle
